Identification of P-type plasma membrane H+-ATPases in common wheat and characterization of TaHA7 associated with seed dormancy and germination
Why this work is in the frame
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Bibliographic record
Abstract
The P-type plasma membrane (PM) H + -ATPases (HAs) are crucial for plant development, growth, and defense. The HAs have been thoroughly characterized in many different plants. However, despite their importance, the functions of HAs in germination and seed dormancy (SD) have not been validated in wheat. Here, we identified 28 TaHA genes ( TaHA1-28 ) in common wheat, which were divided into five subfamilies. An examination of gene expression in strong- and weak-SD wheat varieties led to the discovery of six candidate genes ( TaHA7/-12/-14/-16/-18/-20 ). Based on a single nucleotide polymorphism (SNP) mutation (C/T) in the TaHA7 coding region, a CAPS marker (HA7) was developed and validated in 168 wheat varieties and 171 Chinese mini-core collections that exhibit diverse germination and SD phenotypes. We further verified the roles of the two allelic variations of TaHA7 in germination and SD using wheat mutants mutagenized with ethyl methane sulphonate (EMS) in ‘Jimai 22’ and ‘Jing 411’ backgrounds, and in transgenic Arabidopsis lines. TaHA7 appears to regulate germination and SD by mediating gibberellic acid (GA) and abscisic acid (ABA) signaling, metabolism, and biosynthesis. The results presented here will enable future research regarding the TaHA s in wheat.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it