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Record W4385483847 · doi:10.1371/journal.ppat.1011532

Broadly neutralizing humanized SARS-CoV-2 antibody binds to a conserved epitope on Spike and provides antiviral protection through inhalation-based delivery in non-human primates

2023· article· en· W4385483847 on OpenAlexafffund
Paule Hermet, Benoît Delache, Cécile Hérate, Esther Wolf, Gaily Kivi, Erkki Juronen, Karl Mumm, Eva Žusinaite, Denis E. Kainov, Eve Sankovski, Kai Virumäe, Anu Planken, Andres Merits, Jessica E. Besaw, A.W. Yee, Takefumi Morizumi, Kyumhyuk Kim, Anling Kuo, Asma Berriche, Nathalie Dereuddre‐Bosquet, Quentin Sconosciuti, Thibaut Naninck, Francis Relouzat, Mariangela Cavarelli, Mart Ustav, Derek J. Wilson, Oliver P. Ernst, Andres Männik, R. Legrand, Mart Ustav

Bibliographic record

VenuePLoS Pathogens · 2023
Typearticle
Languageen
FieldMedicine
TopicSARS-CoV-2 and COVID-19 Research
Canadian institutionsAmgen (Canada)University of TorontoYork University
FundersDivision of Microbiology and Infectious Diseases, National Institute of Allergy and Infectious DiseasesNational Institute of Allergy and Infectious DiseasesNatural Sciences and Engineering Research Council of CanadaCanadian Institutes of Health ResearchCHIST-ERACanada Excellence Research Chairs, Government of CanadaAgence Nationale de la RechercheFondation Bettencourt SchuellerEttevõtluse Arendamise SihtasutusNational Institutes of HealthEesti TeadusagentuurOffice of ScienceYork UniversityStyrelsen för Internationellt UtvecklingssamarbeteArgonne National LaboratoryU.S. Department of Energy
KeywordsAntibodyEpitopeMonoclonal antibodyVirologyNeutralizing antibodyImmunologyMedicineViral loadSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)PandemicBiologyCoronavirus disease 2019 (COVID-19)VirusDiseaseInfectious disease (medical specialty)Internal medicine

Abstract

fetched live from OpenAlex

The COVID-19 pandemic represents a global challenge that has impacted and is expected to continue to impact the lives and health of people across the world for the foreseeable future. The rollout of vaccines has provided highly anticipated relief, but effective therapeutics are required to further reduce the risk and severity of infections. Monoclonal antibodies have been shown to be effective as therapeutics for SARS-CoV-2, but as new variants of concern (VoC) continue to emerge, their utility and use have waned due to limited or no efficacy against these variants. Furthermore, cumbersome systemic administration limits easy and broad access to such drugs. As well, concentrations of systemically administered antibodies in the mucosal epithelium, a primary site of initial infection, are dependent on neonatal Fc receptor mediated transport and require high drug concentrations. To reduce the viral load more effectively in the lung, we developed an inhalable formulation of a SARS-CoV-2 neutralizing antibody binding to a conserved epitope on the Spike protein, ensuring pan-neutralizing properties. Administration of this antibody via a vibrating mesh nebulization device retained antibody integrity and resulted in effective distribution of the antibody in the upper and lower respiratory tract of non-human primates (NHP). In comparison with intravenous administration, significantly higher antibody concentrations can be obtained in the lung, resulting in highly effective reduction in viral load post SARS-CoV-2 challenge. This approach may reduce the barriers of access and uptake of antibody therapeutics in real-world clinical settings and provide a more effective blueprint for targeting existing and potentially emerging respiratory tract viruses.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.036
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.085
GPT teacher head0.375
Teacher spread0.290 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations12
Published2023
Admission routes2
Has abstractyes

Explore more

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