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Record W4385542251 · doi:10.1101/2023.08.03.551808

The ketamine metabolite ( <i>2R,6R</i> )-hydroxynorketamine rescues hippocampal mRNA translation, synaptic plasticity and memory in mouse models of Alzheimer’s disease

2023· preprint· en· W4385542251 on OpenAlexaff
Felipe C. Ribeiro, Danielle Cozachenco, Elentina K. Argyrousi, Agnieszka Staniszewski, Shane Wiebe, Joao D. Calixtro, Rubens Soares‐Neto, Aycheh Al‐Chami, Fatema El Sayegh, Sara Bermudez, Emily Arsenault, Marcelo Cossenza, Jean‐Claude Lacaille, Karim Nader, Hongyu Sun, Fernanda G. De Felice, Mychael V. Lourenco, Ottavio Arancio, Argel Aguilar‐Valles, Nahum Sonenberg, Sérgio T. Ferreira

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2023
Typepreprint
Languageen
FieldNeuroscience
TopicTryptophan and brain disorders
Canadian institutionsUniversité de MontréalCarleton UniversityQueen's UniversityCentre for Research on Brain Language and MusicMcGill University
FundersNational Institutes of HealthNational Institute of Neurological Disorders and StrokeInstituto Nacional de Ciência e Tecnologia de Neurociência TranslacionalFundação Carlos Chagas Filho de Amparo à Pesquisa do Estado do Rio de JaneiroUniversidade Federal do Rio de JaneiroAlzheimer's AssociationInternational Brain Research OrganizationInstituto SerrapilheiraConselho Nacional de Desenvolvimento Científico e TecnológicoCompany of BiologistsAlzheimer's SocietyAmerican Society for Biochemistry and Molecular BiologyInternational Society for Neurochemistry
KeywordsLong-term potentiationHippocampal formationSynaptic plasticitymTORC1NeuroscienceMemory consolidationP70-S6 Kinase 1PI3K/AKT/mTOR pathwayHippocampusBiologyRibosomal protein s6Signal transductionCell biologyBiochemistry

Abstract

fetched live from OpenAlex

Abstract Impaired synaptic plasticity and progressive memory deficits are major hallmarks of Alzheimer’s disease (AD). Hippocampal mRNA translation, required for memory consolidation, is defective in AD. Here, we show that systemic treatment with ( 2R,6R )- hydroxynorketamine (HNK), an active metabolite of the antidepressant ketamine, prevented deficits in hippocampal mRNA translation, long-term potentiation (LTP) and memory induced by AD-linked amyloid-β oligomers (AβOs) in mice. HNK activated hippocampal extracellular signal-regulated kinase 1/2 (ERK1/2), mechanistic target of rapamycin (mTOR), and p70S6 kinase 1 (S6K1)/ribosomal protein S6 (S6), which promote protein synthesis and synaptic plasticity. Stimulation of S6 phosphorylation by HNK was mTORC1-dependent, while rescue of hippocampal LTP and memory in HNK-treated AβO-infused mice was ERK1/2-dependent and, partially, mTORC1- dependent. Remarkably, treatment with HNK corrected LTP and memory deficits in aged APP/PS1 mice. Transcriptomic analysis further showed that HNK rescued signaling pathways that are aberrant in APP/PS1 mice, including inflammatory and hormonal responses, and programmed cell death. Taken together, our findings demonstrate that HNK induces signaling and transcriptional responses that correct deficits in hippocampal synaptic plasticity and memory in AD mouse models. These results raise the prospect that HNK could serve as a therapeutic to prevent or reverse memory decline in AD.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.003

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.040
GPT teacher head0.240
Teacher spread0.200 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

Explore more

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