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Record W4385728061 · doi:10.3389/fmicb.2023.1175679

HTLV-1 p12 modulates the levels of prion protein (PrPC) in CD4+ T cells

2023· article· en· W4385728061 on OpenAlexaff
Isabela Silva de Castro, Alessandra Granato, Rafael M. Mariante, Marco Antônio Lima, Ana Claúdia Celestino Bezerra Leite, Otávio de Melo Espíndola, Cynthia A. Pise-Masison, Genoveffa Franchini, Rafael Linden, Juliana Echevarria‐Lima

Bibliographic record

VenueFrontiers in Microbiology · 2023
Typearticle
Languageen
FieldImmunology and Microbiology
TopicT-cell and Retrovirus Studies
Canadian institutionsHospital for Sick Children
FundersUniversidade Federal do Rio de Janeiro
KeywordsTropical spastic paraparesisBiologyImmune systemWestern blotFlow cytometryDownregulation and upregulationInflammationVirologyImmunologyMyelopathyGeneGenetics

Abstract

fetched live from OpenAlex

Introduction Infection with human T cell lymphotropic virus type 1 (HTLV-1) is endemic in Brazil and is linked with pro-inflammatory conditions including HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP), a chronic neuroinflammatory incapacitating disease that culminates in loss of motor functions. The mechanisms underlying the onset and progression of HAM/TSP are incompletely understood. Previous studies have demonstrated that inflammation and infectious agents can affect the expression of cellular prion protein (PrP C ) in immune cells. Methods Here, we investigated whether HTLV-1 infection affected PrP C content in cell lines and primary CD4 + cells in vitro using flow cytometry and western blot assays. Results We found that HTLV-1 infection decreased the expression levels of PrP C and HTLV-1 Orf I encoded p12, an endoplasmic reticulum resident protein also known to affect post-transcriptionally cellular proteins such as MHC-class I and the IL-2 receptor. In addition, we observed a reduced percentage of CD4 + T cells from infected individuals expressing PrP C , which was reflected by IFN type II but not IL-17 expression. Discussion These results suggested that PrP C downregulation, linked to both HTLV-1 p12 and IFN-γ expression in CD4 + cells, may play a role in the neuropathogenesis of HTLV-1 infection.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.043
Threshold uncertainty score0.634

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.001
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.212
Teacher spread0.201 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2023
Admission routes1
Has abstractyes

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