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Record W4385813796 · doi:10.1016/j.ccell.2023.07.013

Integrative multi-omic cancer profiling reveals DNA methylation patterns associated with therapeutic vulnerability and cell-of-origin

2023· article· en· W4385813796 on OpenAlexaff
Wen-Wei Liang, Rita Jui-Hsien Lu, Reyka G. Jayasinghe, Steven M. Foltz, Eduard Porta‐Pardo, Yifat Geffen, Michael C. Wendl, Rossana Lazcano, Iga Kołodziejczak, Yizhe Song, Akshay Govindan, Elizabeth G. Demicco, Xiang Li, Yize Li, Sunantha Sethuraman, Samuel Payne, David Fenyö, Henry Rodriguez, Maciej Wiznerowicz, Hui Shen, D.R. Mani, Karin Rodland, Alexander J. Lazar, Ana I. Robles, Li Ding, François Aguet, Yo Akiyama, Eunkyung An, Shankara Anand, Meenakshi Anurag, Özgün Babur, Jasmin Bavarva, Chet Birger, Michael J. Birrer, Anna Calinawan, Lewis C. Cantley, Song Cao, Steve Carr, Michele Ceccarelli, Daniel Chan, Arul M. Chinnaiyan, Hanbyul Cho, Shrabanti Chowdhury, Marcin Cieślik, Karl R. Clauser, Antonio Colaprico, Daniel Cui Zhou, Felipe da Veiga Leprevost, Corbin Day, Marcin J. Domagalski, Yongchao Dou, Brian Druker, Nathan Edwards, Matthew J. Ellis, Myvizhi Esai Selvan, Alicia Francis, Gad Getz, Michael A. Gillette, Tania Gonzalez Robles, Sara J.C. Gosline, Zeynep H. Gümüş, David I. Heiman, Tara Hiltke, Runyu Hong, Galen Hostetter, Yingwei Hu, Chen Huang, Emily M. Huntsman, Antonio Iavarone, Eric J. Jaehnig, Scott Jewel, Jiayi Ji, Wen Jiang, Jared L. Johnson, Lizabeth Katsnelson, Karen A. Ketchum, Karsten Krug, Chandan Kumar‐Sinha, Jonathan T. Lei, Yuxing Liao, Caleb M. Lindgren, Tao Liu, Wenke Liu, Weiping Ma, Fernanda Martins Rodrigues, Wilson McKerrow, Mehdi Mesri, Alexey I. Nesvizhskii, Chelsea J. Newton, Robert Oldroyd, Gilbert S. Omenn, Amanda G. Paulovich, Francesca Petralia, Pietro Pugliese, Boris Reva, Kelly V. Ruggles, Dmitry Rykunov, Shankha Satpathy, Sara R. Savage, Eric E. Schadt, Michael Schnaubelt, Tobias Schraink, Zhiao Shi, Dick Smith, Xiaoyu Song, Vasileios Stathias, Erik Storrs, Jimin Tan, Nadezhda V. Terekhanova, Ratna R. Thangudu, Mathangi Thiagarajan, Nicole Tignor, Joshua Wang, Liang-Bo Wang, Pei Wang, Ying Wang, Bo Wen, Yige Wu, Lijun Yao, Tomer M. Yaron, Xinpei Yi, Bing Zhang, Hui Zhang, Qing Zhang, Xu Zhang, Zhen Zhang, Daniel W. Chan, Saravana M. Dhanasekaran, Stephan C. Schürer, Richard D. Smith, Matthew A. Wyczalkowski

Bibliographic record

VenueCancer Cell · 2023
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicEpigenetics and DNA Methylation
Canadian institutionsUniversity of TorontoMount Sinai Hospital
FundersNational Cancer InstituteNational Institutes of HealthU.S. Department of Health and Human Services
KeywordsBiologyEpigeneticsDNA methylationMethylationCancer researchReprogrammingCellGeneticsGeneGene expression

Abstract

fetched live from OpenAlex

DNA methylation plays a critical role in establishing and maintaining cellular identity. However, it is frequently dysregulated during tumor development and is closely intertwined with other genetic alterations. Here, we leveraged multi-omic profiling of 687 tumors and matched non-involved adjacent tissues from the kidney, brain, pancreas, lung, head and neck, and endometrium to identify aberrant methylation associated with RNA and protein abundance changes and build a Pan-Cancer catalog. We uncovered lineage-specific epigenetic drivers including hypomethylated FGFR2 in endometrial cancer. We showed that hypermethylated STAT5A is associated with pervasive regulon downregulation and immune cell depletion, suggesting that epigenetic regulation of STAT5A expression constitutes a molecular switch for immunosuppression in squamous tumors. We further demonstrated that methylation subtype-enrichment information can explain cell-of-origin, intra-tumor heterogeneity, and tumor phenotypes. Overall, we identified cis-acting DNA methylation events that drive transcriptional and translational changes, shedding light on the tumor's epigenetic landscape and the role of its cell-of-origin.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.094
Threshold uncertainty score0.497

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.035
GPT teacher head0.323
Teacher spread0.289 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations104
Published2023
Admission routes1
Has abstractyes

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