Synthesis of a Novel Symmetrical Photocleavable Group for the Efficient Capture and Release of Cysteine Sulfenic Acids on Oxidized Proteins From Streptavidin Beads
Bibliographic record
Abstract
Cysteine sulfenic acids are thought to be key intermediates in protein oxidation but are difficult to study due to their high reactivity and transient nature. The beneficial properties of these oxidative events have been underexplored due to earlier studies primarily focusing on cell damage and death; however, increasing evidence suggests various physiological processes rely on these oxidative modifications. Previous work has shown that bicyclo[6.1.0]non-4-ynes (BCNs) can label sulfenic acids with high reaction kinetics via a pericyclic reaction, while also enabling copper-free click chemistry, allowing for a biotin-azide tag to be installed. However, due to the strong biotin- streptavidin interaction, these probes suffer from poor protein recovery and sub-optimal performance during mass-spectrometric analysis, respectively. Efforts towards incorporating the BCNs into a novel symmetrical photocleavable group based on 1,4-naphthoquinone, or their use in combination with photocleavable biotin-azide are proposed to improve upon these limitations and are presented in this thesis.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.002 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".