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Record W4386775692 · doi:10.1139/cjps-2022-0252

Evaluation of <i>Vicia faba</i> plant genotypes using non-destructive vibrational molecular spectroscopic technique (ATR-FT/IR): molecular structure profiles of protein in relation to nutritional characteristics

2023· article· en· W4386775692 on OpenAlexafffundvenue
María E. Rodríguez Espinosa, Peiqiang Yu

Bibliographic record

VenueCanadian Journal of Plant Science · 2023
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicAgricultural pest management studies
Canadian institutionsUniversity of Saskatchewan
FundersNatural Sciences and Engineering Research Council of CanadaSaskatchewan Pulse Growers
KeywordsVicia fabaFourier transform infrared spectroscopyTanninChemistryProtein secondary structureAmideInfrared spectroscopyAbsorbanceInfraredAnalytical Chemistry (journal)Food scienceBiochemistryChromatographyBiologyBotanyOrganic chemistry

Abstract

fetched live from OpenAlex

This study aimed to evaluate the impact of genotypes and tannin levels on the nutritional characteristics of faba bean seeds and their relation to protein molecular structure profiles using Fourier transform infrared spectroscopy (FTIR). Eight genotypes with low tannin (LT, n = 3) and normal tannin (NT, n = 5) levels were studied. Standard methods of analysis and NRC 2001 were used to obtain chemical and energy parameters. The protein molecular spectral study was performed using a spectrometer JASCO FT/IR–4200. Molecular features were analyzed in the mid-infrared region at ~4000–800 cm −1 and protein-related functional groups were obtained using OMNIC software. Results showed higher ( P &lt; 0.01) soluble crude protein (SCP), rapidly degradable protein fraction (PA2), and intestinal digestibility of bypass protein (dIDP) in LT than NT. The slowly degradable protein fraction (PB2) was higher in NT ( P &lt; 0.01) compared to LT. Higher absorbance was observed in spectral peaks related to amide I, amide II, and β-sheet in NT ( P &lt; 0.05) compared to LT. The area ratio of amide I to amide II and the height ratio of α-helix to β-sheet were higher in LT compared to NT ( P &lt; 0.01). This study provides a better understanding of associations between structure and nutritional characteristics in faba bean genotypes with different tannin levels.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.762
Threshold uncertainty score0.230

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.018
GPT teacher head0.232
Teacher spread0.214 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2023
Admission routes3
Has abstractyes

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