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Record W4387103580 · doi:10.1101/2023.09.20.558508

An RNA foundation model enables discovery of disease mechanisms and candidate therapeutics

2023· preprint· en· W4387103580 on OpenAlexaff
Albi Celaj, Alice Jiexin Gao, Tammy Lau, Erle M. Holgersen, Alston Lo, Varun Lodaya, C. B. Cole, Robert E. Denroche, Carl Spickett, Omar Wagih, Pedro O. Pinheiro, Parth Vora, Pedrum Mohammadi‐Shemirani, Steve Chan, Zach Nussbaum, Xi Zhang, Helen He Zhu, Easwaran Ramamurthy, Bhargav Kanuparthi, Michael A. Iacocca, Diane Ly, Ken J. Kron, Marta Verby, Kahlin Cheung-Ong, Zvi Shalev, Brandon Vaz, Sakshi Bhargava, Farhan Yusuf, Sharon Samuel, Sabriyeh Alibai, Zahra Baghestani, Xinwen He, Kirsten Krastel, Oladipo Oladapo, Amrudha Mohan, Arathi Shanavas, Magdalena Bugno, Jovanka Bogojeski, Frank W. Schmitges, Carolyn Kim, Solomon Grant, Rachana Jayaraman, Tehmina Masud, Amit G. Deshwar, Shreshth Gandhi, Brendan J. Frey

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2023
Typepreprint
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA Research and Splicing
Canadian institutionsOntario Genomics
Fundersnot available
KeywordsRNARNA splicingComputational biologyBiologyPolyadenylationIntronExonGeneGeneticsNon-coding RNAAlternative splicing

Abstract

fetched live from OpenAlex

Abstract Accurately modeling and predicting RNA biology has been a long-standing challenge, bearing significant clinical ramifications for variant interpretation and the formulation of tailored therapeutics. We describe a foundation model for RNA biology, “BigRNA”, which was trained on thousands of genome-matched datasets to predict tissue-specific RNA expression, splicing, microRNA sites, and RNA binding protein specificity from DNA sequence. Unlike approaches that are restricted to missense variants, BigRNA can identify pathogenic non-coding variant effects across diverse mechanisms, including polyadenylation, exon skipping and intron retention. BigRNA accurately predicted the effects of steric blocking oligonucleotides (SBOs) on increasing the expression of 4 out of 4 genes, and on splicing for 18 out of 18 exons across 14 genes, including those involved in Wilson disease and spinal muscular atrophy. We anticipate that BigRNA and foundation models like it will have widespread applications in the field of personalized RNA therapeutics.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.003
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Simulation or modeling · Consensus signal: Simulation or modeling
GenreCandidate signal: Methods · Consensus signal: Methods
Teacher disagreement score0.004
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.003
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0010.001
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0030.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.020
GPT teacher head0.262
Teacher spread0.241 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designSimulation or modeling
Domainnot available
GenreMethods

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations30
Published2023
Admission routes1
Has abstractyes

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