Partial C-terminal truncation of Bx and Dy high molecular weight glutenin subunits after conserved aspartate
Bibliographic record
Abstract
The composition of polymeric gluten proteins and interaction with non-covalently associated gliadins define the functional utility of a flour. This composition is dictated by the timing of cellular and genetic events in the plant and the developing seed. This interplay and its effect on gluten functionality is poorly understood and could provide a new opportunity for improving wheat quality in the future. Truncated proteoforms of high-molecular weight glutenin subunits (HMWGS) were observed for Bx and Dy subtypes across 49 Canadian bread wheat and 16 durum varieties. The relative abundances of these variants ranged from 7.5% to 37.7% and abundances of Bx and Dy variants were highly correlated (r2 = 0.85). HMWGS Bx7* and Dy10 were purified and the mass difference was shown to be from the loss of a C-terminal hexapeptide after a conserved aspartate residue. This aspartate was present at the ω-7 position in >90% of HMWGS accessions and the C-terminal sequence was highly conserved. Importantly, all HMWGS subtypes contain the same conserved sequences, but truncated variants were never observed for By, Dx and Ax HMWGS. The potential impact of these truncated HMWGS on gluten form and function is discussed.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".