OC 48.4 The Throm-PED Registry: Epidemiology and Treatment of Adolescent Thrombosis
Bibliographic record
Abstract
Background: The choroid plexus and ependyma are predominantly affected by extravasated blood upon intraventricular hemorrhage (IVH) in preterm infants causing the breakdown of the blood-brain-barrier.Hence, the impact of hemorrhage on choroid plexus epithelium is critical in potential brain damage following IVH.Aims: We characterized the cellular, inflammatory, and oxidative responses in human choroid plexus epithelial cells (HCPEpiC) exposed to heme in vitro.Methods: Cultured HCPEpiC were treated with heme (1-50 μM) for 1-24 hours.The NF-κB pathway activation was investigated by fluorescence microscopy, while reactive oxygen species (ROS) were measured intracellularly using CM-H2DCFDA-based assay.Expression of related signaling molecules and secreted proteins was studied by western blotting and ELISA.The blocking effects of albumin/hemopexin were also observed.To analyze the consequence of heme on global RNA expression in choroid plexus, transcriptome and miRNA pattern were investigated by RNA-sequencing and TaqMan OpenArray, respectively.ClueGO and miRTarbase databases were used for pathway and network analysis.Results: Increased ROS production ( p < 0.001) and induced NF-κB pathway activation without cell death was shown after treatment with 25 μM of heme.Out of 1529 significantly altered genes, 231 showed more than 2-fold upregulation and 106 were downregulated in association with the activation of inflammatory pathways (IL8, IL6, ICAM1), TLR4 signaling (IL1B) and chemical stress (HMOX1, SOD2).Significantly elevated concentrations of IL-8, IL-6, IL-1β and ICAM-1 were detected via elevated expression of HMOX1, NQO1 and SOD2 proteins in response to heme.These cellular events were prevented by albumin/hemopexin treatment.Among 355 detected miRNAs, there were 25 significantly downregulated and 10 upregulated miRNAs vs. controls.Based on the network analysis, downregulated miR-223 was associated with upregulated IL6, while reduced miR-9 and miR-505 resulted in higher SOD2 level.Conclusion(s): Heme acts as a major mediator of the damage of choroid plexus epithelium after IVH, which contribute to neurodevelopmental impairment in preterm infants.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.002 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.002 | 0.004 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.001 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.019 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".