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Transglutaminase 2 Plays a Critical, but Context‐Specific Role in Glioblastoma Proliferation

2016· article· en· W4389033854 on OpenAlexaff
Alina Monteagudo, Soner Gundemir, Abdullah Akbar, Jeffrey W. Keillor, Gail V.W. Johnson

Bibliographic record

VenueThe FASEB Journal · 2016
Typearticle
Languageen
FieldMedicine
TopicBlood properties and coagulation
Canadian institutionsUniversity of Ottawa
FundersNational Institutes of Health
KeywordsNeurosphereContext (archaeology)Cancer researchCell growthBiologyTissue transglutaminaseCell cultureTemozolomideGlioblastomaGeneCellular differentiationGeneticsEnzymeBiochemistry

Abstract

fetched live from OpenAlex

Glioblastoma multiforme (GBM) is the most aggressive and common form of adult primary brain tumors. GBMs are characterized by resistance to chemotherapy and radiation, and exhibit a high propensity for reoccurrence. Therefore, it is imperative to define molecular mechanisms that contribute to GBM tumor survival and proliferation. One possible protein that may contribute to GBM proliferation is the multifunctional protein transglutaminase 2 (TG2). TG2 has been shown to play a critical role in proliferation in other types of cancers, and therefore we investigated its role in GBMs. Given the heterogeneity of GBM tumors, we used various GBM cell lines as well as non‐serum containing GBM neurospheres to determine if the role of TG2 in GBMs was broadly applicable, or just relevant to certain populations. Additionally, the neurospheres used represent three different GBM subtypes: mesenchymal, proneural and classical. By the use of novel inhibitors and genetic manipulations of TG2, results show that in most, but not all, cell lines TG2 plays a critical role in the proliferative process of GBM cells. Given the heterogeneity of GBM tumors, it is not surprising that TG2 facilitates the proliferative process in only certain GBM subtypes. Nonetheless, as the understanding of signaling mechanisms that contribute to GBM proliferation increases, TG2 inhibitors may in the future be effective therapeutic agents for certain GBMs, as determined by specific genetic determinants. Support or Funding Information NIH RO1 NS065825

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.108
Threshold uncertainty score0.385

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.020
GPT teacher head0.248
Teacher spread0.229 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2016
Admission routes1
Has abstractyes

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