Does amyloid b precursor protein gene expression have a role in diagnosis of biliary atresia?
Bibliographic record
Abstract
Aim of the study: Biliary atresia (BA) is an important cause of surgical jaundice.Although the precise etiology is unknown, b-amyloid (Ab) has been observed around the bile ducts in BA livers.It is unclear whether Ab plays a role in the pathogenesis of this disease.This study aims to assess the amyloid b precursor protein (APP) gene expression in infants with BA in comparison with other causes of neonatal cholestasis.This could help explore the role of Ab in the pathogenesis and diagnosis of BA.Material and methods: A prospective study was conducted at the outpatient clinic of Paediatric Hepatology, Gastroenterology, and Nutrition Department, National Liver Institute, Menoufia University, Shebin El Kom, Menoufia, Egypt during the period March 2022 to December 2022.Clinical data were gathered and laboratory and radiological investigations were conducted including b precursor protein gene expression measured in liver biopsies of the three groups using quantitative real-time PCR (qPCR).Results: Gene expression of APP was considerably higher in the BA group (p = 0.0001) compared to neonatal cholestasis (NC) patients.Gamma glutamyl transferase (GGT) and APP had a positive correlation (p = 0.001).No significant association was found between APP and fibrosis.APP was noticeably higher in BA than NC other than BA.Also, APP in BA was higher (statistically significant, p = 0.0001) than the control.There was no statistically significant difference among NC, BA, and control groups regarding APP (p = 0.07).Both males and females did not show significant differences as regards APP (p = 0.851).Age did not have a statistically significant correlation with APP (p = 0.532).Also, there were no correlations between APP and alkaline phosphatase (ALP), aspartate transaminase (AST), or total bilirubin (TB) (p > 0.05).Conclusions: We concluded that the development and identification of BA may depend on the liver expression of serum APP.Surgeons may be able to carry out early intraoperative cholangiography for BA confirmation if the combination of APP with GGT and other hepatic function parameters exhibits a high predictive potential as a diagnostic test for BA.To evaluate this hypothesis, more research with sizable sample numbers is necessary.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.004 | 0.014 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.002 | 0.001 |
| Science and technology studies | 0.001 | 0.002 |
| Scholarly communication | 0.002 | 0.003 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.003 | 0.003 |
| Insufficient payload (model declined to judge) | 0.004 | 0.003 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".