Isolation, Morphological And Biochemical Identification Of Endophytic Fungi From Moringa oleifera And Its Antimicrobial Potential
Bibliographic record
Abstract
The present study was aimed at isolating endophytic fungi from the leaves of M. oleifera and analyzing its antimicrobial potential. In this study, the fungal endophyte Acremonium sp. was isolated identified through morphological and biochemical analysis. In IAA the strain produced 55.27 ± 3.00 μgml-1 IAA without exogenous tryptophan application (control) in the culture medium. The addition of 1mgml-1 tryptophan to the culture medium increased the IAA production to 76 ± 2.6 μgml-1 The production of siderophore was performed in the absence and presence of an iron source in the medium. The strain was cultured in the minimal media supplemented with different Fe(III) citrate concentrations, i.e., 0 μM, 0.25 μM, 2.0 μM, and 4.0 μM. The isolated fungi showed high siderophore production when cultured in a medium without Fe(III) citrate. Siderophore production found to be 40.00 ± 0.4 (psu) in the culture medium without the addition of Fe(III) citrate.. Siderophore production was slightly reduced , i.e., 33 ± 0.3 (psu) at 0.25 μM Fe(III) citrate in the culture medium. However, a significant reduction in the production of the siderophore was observed when Fe (III) citrate content was raised to 2.0 μM and 4.0 μM. In case of bacterial strains it was observed that maximum activity was observed against P. aeruginosa at 80 µg/ml (IZ-20mm) while minimum at 20 µg/ml against E. coli (10mm) but at 40 and 60 µg/ml activity was at par (IZ-14mm) while S. aureus was found to be partially resistant. In case of antifungal it was observed that when extract of PD 20 was tested maximum activity was observed at 80 µg/ml against T. reesei (IZ-22mm) while C. albicans was found to be partially resistant as initially no activity was observed against it. Against P. funiculosum no activity was observed at 20 µg/ml but with increase dose level activity increased.Therefore, we conclude that the isolated Acremonium sp. should be further investigated before utilization as a biocontrol agent and plant growth stimulator
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".