Signalling pathway associated with discharge of cnidocyst induced by reduced glutathione in <i>Hydra plagiodesmica</i> (Dioni, 1968)
Bibliographic record
Abstract
In the present work, we study the effect of different stimuli (living preys, its homogenate, and reduced glutathione (GSH)) on the discharge of desmonemes in the freshwater cnidarian, Hydra plagiodesmica (Dioni, 1968) (Cnidaria: Hydrozoa). Once confirmed their stimulatory effect, we choose GSH to analyze the relevance of Ca2+, the probable identity of the sensory receptor molecule, and the specific signalling pathway driving the discharge mechanism. Our results show that not only mechanical, but also chemical stimuli by itself may stimulate the discharge of desmonemes. Using calcium chelators, we verify that the discharge mechanism triggered by GSH is dependent on calcium. By mean of drugs that inhibit proteins pertaining to canonical signalling pathways, including GPCRs, we show that GSH causes the influx of Ca2+ via L-type voltage sensitive calcium channel (VGCC), and its release by mean of endoplasmic reticulum calcium channels. Moreover, our results suggest that the putative receptor for GSH is a GPCR coupled to Gq, which produces IP3 and Ca2+ as second messengers. Finally, we show that the genome of Hydra vulgaris (Pallas, 1766) predicts the existence of the different subunits of L-type VGCC, showing a high level of conservation when compared with other groups of Metazoa.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".