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Record W4389386953 · doi:10.21203/rs.3.rs-3613980/v1

Unlocking the potential of biofilm- forming plant growth-promoting rhizobacteria for growth and yield enhancement in wheat (Triticum aestivum L.): Results from In vitro and in vivo studies

2023· preprint· en· W4389386953 on OpenAlexaff
Munazza Rafique, Muhammad Naveed, Muhammad Zahid Mumtaz, Abid Niaz, Saud Alamri, Manzer H. Siddiqui, Zulfiqar Ali, Abdul Naman, Sajid Rehman, Martin Brtnický, Adnan Mustafa, Muhammad Qandeel Waheed

Bibliographic record

VenueResearch Square · 2023
Typepreprint
Languageen
FieldAgricultural and Biological Sciences
TopicPlant-Microbe Interactions and Immunity
Canadian institutionsBiotechnology Research Institute
FundersKing Saud University
KeywordsRhizobacteriaBiofilmAxenicBiologyRhizosphereShootBiomass (ecology)PhosphorusBacteriaBotanyHorticultureFood scienceAgronomyChemistry

Abstract

fetched live from OpenAlex

Abstract Plant growth-promoting rhizobacteria (PGPR) boosts agricultural productivity and alleviates environmental stresses by forming biofilms under natural climatic conditions. In the past few years, microorganisms in biofilm have gained impetus for efficient root colonization. The current work aims to characterize biofilm-associated rhizobacteria for wheat growth and yield enhancement. In this study native rhizobacteria were isolated from the wheat rhizosphere and ten isolates were characterized for plant growth promoting traits and biofilm production under axenic conditions. Among these ten isolates, five potential biofilm-producing plant growth-promoting rhizobacteria on the basis of invitro plant growth promoting trait assays were further tested under controlled and field conditions on wheat growth and yield attributes. Surface-enhanced Raman spectroscopy (SERS) spectra further revealed that biochemical contents of biofilm produced by selected bacterial PGPR strains are associated with proteins, carbohydrates, lipids, amino acids and DNA/RNA. Inoculated plants in growth chamber resulted in longer roots, shoots, and increase in fresh biomass than controls. Similarly, significant increases in plant height (up to 13.3, 16.7%), grain yield (up to 29.6, 46.9%), number of tillers (up to 18.7, 34.8%), nitrogen contents (up to 58.8, 48.1%), and phosphorus contents (up to 63.0, 51.0%) in grains were seen in both pot and field trials, respectively. The two most promising biofilm-producing isolates were identified through 16s rRNA partial gene sequencing as Brucella sp. (BF10), Lysinibacillus macroides (BF15). Moreover, leaf pigmentation and relative water contents increased in all treated plants. Taken together, our results revealed that biofilm forming PGPR can boost crop productivity for sustainable agriculture.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.002

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.095
GPT teacher head0.334
Teacher spread0.239 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2023
Admission routes1
Has abstractyes

Explore more

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