APE1 associates with 60S ribosomes and tRNAs and regulates the expression of IGF2BP1
Bibliographic record
Abstract
Abstract Apurinic/apyrimidinic endonuclease 1 (APE1), a multifunctional protein known for its DNA repair function and redox regulation, is often found overexpressed in cancers. APE1 can be found in the nucleus, cytoplasm and secreted extracellularly. APE1 subcellular distribution in the cytoplasm is frequently reported in various types of cancer but the biological significance remains unknown. In this study, APE1 in the cytoplasm of HepG2 cells was investigated using various techniques including microscopy, differential centrifugation, sucrose gradient fractionation and CL-IP. APE1 was found to associate with 60S ribosomes and tRNAs under native conditions, suggesting it may have a specific function in the translational machinery. Knockdown of APE1 in HepG2 cells led to increased protein expression of IGF2BP1 as well as enhanced HepG2 cell migration, suggesting that APE1 can act as a tumor suppressor in this cell line model of hepatocellular carcinoma. When APE1 was depleted, the translation of a reporter construct containing the 3’UTR of IGF2BP1 was enhanced. This study provides evidence in support of the role of cytoplasmic APE1 in the control of IGF2BP1 protein translation and sheds light on the potential novel function of cytoplasmic APE1.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".