O0043 SOYBEAN LECITHIN IMPROVES BILIARY SECRETION AND PROTECTS AGAINST LIVER FIBROSIS IN CHOLESTATIC MDR2 KNOCK‐OUT MICE
Bibliographic record
Abstract
Introduction: Soybean lecithin has been shown to decrease the development of fibrosis in alcohol- or CCl4-induced liver disease in animals. The aim of the study was to investigate the antifibrotic properties of lecithin in Mdr2 KO mice, a model of cholestatic liver disease in which animals rapidly develop fibrosis when receiving cholic acid. Methods: After weaning, Mdr2 KO mice were divided in 4 separated diet groups of 5–6 animals: control diet (C), control diet + 0.025% cholic acid (CA), soybean lecithin containing diet (L), and soybean lecithin containing diet + 0.025% cholic acid (L+CA). After 2 weeks, blood sample and bile were collected, then animals were sacrificed and liver was excised. Biliary concentrations of bile acids, cholesterol and phospholipid, liver lipid content, and serum liver enzyme levels (ALAT, ALP, GGT) were measured. Histological examination of the liver was performed, using cytokeratin immunostaining for bile duct proliferation, alpha-smooth muscle actin immunostaining for fibroblast activation, BrdU immunostaining for cellular proliferation, and Sirius red staining for fibrosis. A quantification was performed using image analysis. Results: In mice receiving cholic acid (CA), body weight decreased and liver weight increased compared with other groups. Serum liver enzyme levels were markedly increased in mice receiving cholic acid (CA) but remained similar with control (C) when these animals were fed with lecithin (L+CA) (Table1). Bile flow (6.4 + 0.8 vs 4.9 + 1.7 ul/min/100gBW), and secretion of bile acids (211 + 77 vs 154 + 65 mmol/min/100gBW), phospholipid (0.19 + 0.1 vs 0.1 + 0.06 mmol/min/100gBW) and cholesterol (1.35 + 0.8 vs 0.77 + 0.63 mmol/min/100gBW) were increased in group L+CA versus group CA (p<0.01). Liver content of lipids was similar in group CA compared with group C, and was decreased in animals receiving lecithin (groups L and L+CA). Bile duct proliferation, fibroblast activation, cell proliferation in portal tracts, and liver fibrosis, were significantly increased in mice receiving cholic acid (group CA) compared with controls (group C), but remained similar to controls when animals receiving cholic acid were supplemented with lecithin (group L+CA). Conclusion: This study demonstrates that soybean lecithin improves biliary secretion and liver pathology in the Mdr2 KO mice model. These observations lead to propose that clinical trials with lecithin could take place in human cholestatic diseases.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.004 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".