P0029 PP PROBIOTICS REDUCE COLONIC HYPERPLASIA AND MUCOSAL INFLAMMATION FOLLOWING CITROBACTER RODENTIUM INFECTION OF MICE
Bibliographic record
Abstract
Introduction: C. rodentium is a murine pathogen in mice, which causes both attaching and effacing lesions and colonic hyperplasia in infected mice. The aims of this study were to determine the efficacy of a live probiotic mixture of Lactobacillus rhamnosus and L. acidophilus (Lacidofil®), and to delineate the protective abilities of these probiotics against C. rodentium-induced changes in the colonic mucosa. Methods: Forty female C57BL/6 mice were divided into 5 groups: group A (N=5) was fed sterile water; group B (N=5) administered probiotics (109 CFU/ml) in sterile drinking water; group C (N=5) was fed maltodextrin alone in sterile drinking water; group D (N=5) was challenged orogastrically with C. rodentium (107 CFU/0.1ml) and then fed sterile water; group E (N=10) was pre-treated with maltodextrin in drinking water for one week prior to C. rodentium challenge; and group F (N=10) was pre-treated with probiotics (109 CFU/ml) for one week prior to challenge with C. rodentium. C. rodentium colonization was determined by plating rectal swabs and luminal contents onto McConkey agar plates. At sacrifice, 10 days after infection, colonic tissues were collected for histology and transmission electron microscopy (TEM). Results: Treatment with a mixture of viable probiotics reduced the severity of colonic colonization with C. rodentium, prevented C. rodentium-induced colonic hyperplasia (crypt height of infected group = 212 +/− 8.5mum: crypt height of treated group = 131 +/− 8.4mum; ANOVA p<0.001) and decreased bacterial internalization and cellular damage caused by C. rodentium infection. Conclusion: Viable probiotics provide a safe intervention for reducing the sequelae of C. rodentium infection in mice. Understanding the mechanism of action underlying these beneficial effects will set the stage for determining the efficacy of probiotics in preventing infection by other attaching-effacing enteric pathogens, including enterohemorrhagic Escherichia coli O157:H7 infection in humans.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.001 | 0.001 |
| Insufficient payload (model declined to judge) | 0.003 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".