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Record W4390707083 · doi:10.1096/fj.202301428rr

<scp>CD4</scp> <sup>+</sup> T cell‐derived <scp>IFN</scp> ‐γ and <scp>LIGHT</scp> synergistically upregulate chemokine production from airway smooth muscle cells

2024· article· en· W4390707083 on OpenAlexafffund
Mu‐Yang Zhou, Rui Sun, Rohin Chakraborty, Christina Wang, Anne‐Marie Lauzon, James G. Martin

Bibliographic record

VenueThe FASEB Journal · 2024
Typearticle
Languageen
FieldMedicine
TopicAsthma and respiratory diseases
Canadian institutionsMcGill UniversityMcGill University Health Centre
FundersNatural Sciences and Engineering Research Council of CanadaGovernment of Canada
KeywordsCell biologyChemokineDownregulation and upregulationLymphotoxinCCL5CXCL14CXCL16T cellChemistryCXCL2CXCL9BiologyMolecular biologyCXCL10ImmunologyChemokine receptorInflammationIL-2 receptorCytokineImmune systemBiochemistry

Abstract

fetched live from OpenAlex

Abstract Airway smooth muscle (ASM) remodeling in asthmatic airways may contribute to persistent airflow limitation and airway hyperresponsiveness. CD4 + T cells infiltrate the ASM layer where they may induce a proliferative and secretory ASM cell phenotype. We studied the interaction between activated CD4 + T cells and ASM cells in co‐culture in vitro and investigated the effects of CD4 + T cells on chemokine production by ASM cells. CD4 + T cells induced marked upregulation of C‐X‐C motif chemokine ligands (CXCL) 9, 10, and 11 in ASM cells. Blockade of the IFN‐γ receptor on ASM cells prevented this upregulation. Furthermore, T cell‐derived IFN‐γ and LIGHT (lymphotoxin, exhibits inducible expression and competes with HSV glycoprotein D for binding to herpesvirus entry mediator, a receptor expressed on T lymphocytes) synergize in a dose‐dependent manner to coordinately enhance CXCL9, 10, and 11 expression. The synergistic property of LIGHT was mediated exclusively through the lymphotoxin‐β receptor (LTBR), but not herpes virus entry mediator (HVEM). Disruption of LTBR signaling in ASM cells reduced CXCL9, 10, and 11 production and ASM cell‐mediated CD4 + T cell chemotaxis. We conclude that the LIGHT‐LTBR signaling axis acts together with IFN‐γ to regulate chemokines that mediate lymphocyte infiltration in asthmatics.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.005
Threshold uncertainty score0.015

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0050.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.009
GPT teacher head0.222
Teacher spread0.213 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations4
Published2024
Admission routes2
Has abstractyes

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