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Record W4390751827 · doi:10.1038/s41467-023-44211-0

IgM N-glycosylation correlates with COVID-19 severity and rate of complement deposition

2024· article· en· W4390751827 on OpenAlexaff
Benjamin Haslund-Gourley, Kyra Woloszczuk, Jintong Hou, Jennifer Connors, Gina Cusimano, Matthew R. Bell, Bhavani Taramangalam, Slim Fourati, Nathan Mege, Mariana Bernui, Matthew C. Altman, Florian Krammer, Harm van Bakel, Al Ozonoff, Lauren I. R. Ehrlich, Esther Melamed, Ana Fernández-Sesma, Viviana Simon, Bali Pulendran, Kari C. Nadeau, Mark M. Davis, Grace A. McCoey, Rafick‐Pierre Sékaly, Lindsey R. Baden, Ofer Levy, Joanna Schaenman, Elaine F. Reed, Albert C. Shaw, David A. Hafler, Ruth R. Montgomery, Steven H. Kleinstein, Patrice M. Becker, Alison D. Augustine, Carolyn S. Calfee, David J. Erle, Michael E. DeBakey, David B. Corry, Mark A. Atkinson, Scott C. Brakenridge, Nelson Iván Agudelo Higuita, Jordan P. Metcalf, Catherine L. Hough, William B. Messer, Monica Kraft, Chris Bime, Bjoern Peters, Carly E. Milliren, Caitlin Syphurs, Kerry McEnaney, Brenda Barton, Claudia Lentucci, Mehmet Saluvan, Ana C. Chang, Annmarie Hoch, Albert Marisa, Tanzia Shaheen, Alvin T. Kho, Shanshan Liu, Sanya Thomas, Jing Chen, Maimouna D. Murphy, Mitchell Cooney, Arash Nemati Hayati, Robert W. Bryant, James Abraham, Naresh Doni Jayavelu, Scott Presnell, Tomasz Jancsyk, Cole Maguire, Jingjing Qi, Brian Lee, Denise Esserman, Leying Guan, Jeremy P. Gygi, Shrikant Pawar, Anderson F. Brito, Gabriela K. Fragiadakis, Ravi K. Patel, James A. Overton, Randi Vita, Kerstin Westendorf, Casey P. Shannon, Scott J. Tebbutt, Rama Thyagarajan, Justin F. Rousseau, Dennis Wylie, Todd Triplett, Erna Milunka Kojic, R. Sharon Chinthrajah, Neera Ahuja, Angela J. Rogers, Maja Artandi, Linda N. Geng, George A. Yendewa, Debra Powell, James N. Kim, Brent Simmons, I. Michael Goonewardene, Cecilia M. Smith, Mark G. Martens, Amy C Sherman, Stephen R. Walsh, Nicolas C. Issa, Ramin Salehi‐Rad, Charles S. Dela Cruz, Shelli Farhadian, Akiko Iwasaki, Albert I. Ko, Evan J. Anderson, Aneesh K. Mehta, Jonathan Sevransky, Vicki Seyfert‐Margolis, Aleksandra Leligdowicz, Michael A. Matthay, Jonathan P. Singer, Kirsten N. Kangelaris, Carolyn M. Hendrickson, Matthew F. Krummel, Charles Langelier, Prescott G. Woodruff, Matthew Lee Anderson, Faheem W. Guirgis, Douglas A. Drevets, Brent R. Brown, Sarah Siegel, Zhengchun Lu, Jarrod Mosier, Hiroki Kimura, Bernard Khor, Adeeb Rahman, Daniel Stadlbauer, Jayeeta Dutta, Hui Xie, Seunghee Kim‐Schulze, Ana S. Gonzalez‐Reiche, Adriana van de Guchte, Juan Manuel Carreño, Gagandeep Singh, Ariel Raskin, Johnstone Tcheou, Dominika Bielak, Hisaaki Kawabata, Geoffrey Kelly, Manishkumar Patel, Kai Nie, Temima Yellin, M. Fried, Leeba Sullivan, Sara Morris, Scott F. Sieg, Hanno Steen, Patrick van Zalm, Benoit Fatou, Kevin Mendez, Jessica Lasky‐Su, Scott R. Hutton, Gregory A. Michelotti, Kari E. Wong, Meenakshi Jha, Arthur Viodé, Naama Kanarek, Boryana Petrova, Yujiao Zhao, Steven E. Bosinger, Arun K. Boddapati, Greg K. Tharp, Kathryn L. Pellegrini, Elizabeth Beagle, David Cowan, Sydney Hamilton, Susan Pereira Ribeiro, Thomas Hodder, Lindsey B. Rosen, Serena Lee, Michael R. Wilson, Ravi Dandekar, Bonny D. Alvarenga, Jayant V. Rajan, Walter L. Eckalbar, Andrew Schroeder, Alexandra Tsitsiklis, Eran Mick, Yanedth Sanchez Guerrero, Christina Love, Lenka Maliskova, Michael Adkisson, Nadia Siles, Janelle N. Geltman, Kerin Hurley, Miti Saksena, Deena R. Altman, Komal Srivastava, Lily Eaker, Maria C. Bermúdez‐González, Katherine F. Beach, Levy A. Sominsky, Arman R. Azad, Lubbertus C. F. Mulder, Giulio Kleiner, Alexandra S. Lee, Evan Do, Andrea Fernandes, Monali Manohar, Thomas Hagan, Catherine A. Blish, Hena Din, Jonasel Roque, Samuel Yang, Natalia Sigal, Iris Chang, Heather Tribout, Paul Harris, Mary Consolo, Carolyn Pope Edwards, Edward Lee, Edward H. Lin, Brett Croen, Nicholas C. Semenza, Brandon Rogowski, Nataliya Melnyk, Sara Furukawa, Renee McLin, Pamela Schearer, Julie Sheidy, George P. Tegos, Crystal Nagle, Kinga K. Smolen, Michaël Desjardins, Simon D. van Haren, Xhoi Mitre, Jessica Cauley, Xiaofang Li, Alexandra Tong, Bethany Evans, Christina Montesano, Jose Humberto Licona, Jonathan Krauß, Jun Bai Park Chang, Natalie Izaguirre, Rebecca Rooks, David Elashoff, Jenny Brook, Estefania Ramires‐Sanchez, Megan Llamas, Adreanne Rivera, Claudia Perdomo, Dawn C. Ward, Clara E. Magyar, Jennifer A. Fulcher, Harry Pickering, Subha Sen, Omkar Chaudhary, Andreas Coppi, John Fournier, Subhasis Mohanty, Catherine Muenker, Allison Nelson, Khadir Raddassi, Michael Rainone, William Ruff, Syim Salahuddin, Wade L. Schulz, Pavithra Vijayakumar, Haowei Wang, Elsio A. Wunder, H. P. Young, Jessica E. Rothman, Anna Konstorum, Ernie Chen, Chris Cotsapas, Nathan D. Grubaugh, Xiaomei Wang, Leqi Xu, Hiromitsu Asashima, Laurel Bristow, Laila Hussaini, Kieffer Hellmeister, Hady Samaha, S. Wimalasena, Andrew Cheng, Christine Spainhour, Erin M. Scherer, Brandi Johnson, Amer Bechnak, Caroline Ciric, Lauren T. Hewitt, Erin Carter, Nina McNair, Bernadine Panganiban, Christopher Huerta, Jacob Usher, Tatyana Vaysman, Steven M. Holland, Yumiko Abe‐Jones, Saurabh Asthana, Alexander J. Beagle, Sharvari Bhide, Sidney Carrillo, Suzanna Chak, Rajani Ghale, Ana Gonzalez, Alejandra Jáuregui, Norman L. Jones, Tasha Lea, Deanna Lee, Raphael Lota, Jeff Milush, Viet Nguyen, Logan Pierce, Priya A. Prasad, Arjun A. Rao, Bushra Samad, Cole Shaw, Austin Sigman, Pratik Sinha, Alyssa Ward, Andrew Willmore, Jenny Zhan, Sadeed Rashid, Nicklaus Rodriguez, Kevin Tang, Luz Torres Altamirano, Legna Betancourt, Cindy Curiel, Nicole Sutter, Maria Tercero Paz, Gayelan Tietje-Ulrich, Carolyn Leroux, Neeta Thakur, Joshua Vasquez, Lekshmi Santhosh, Li-Zhen Song, Ebony Nelson, Lyle L. Moldawer, Brittany Borresen, Brittney Roth-Manning, Ricardo Ungaro, Jordan Oberhaus, J. Leland Booth, Lauren A. Sinko, Amanda E. Brunton, Peter E. Sullivan, Matthew Strnad, Zoë L. Lyski, Felicity J. Coulter, Courtney Micheleti, Michelle Conway, David Francisco, Allyson Molzahn, Heidi L. Erickson, Connie Cathleen Wilson, Ron Schunk, Bianca Sierra, Trina Hughes, Holden T. Maecker, Nadine Rouphael, Joann Diray‐Arce, Brian Wigdahl, Michele A. Kutzler, Charles B. Cairns, Elias K. Haddad, Mary Ann Comunale

Bibliographic record

VenueNature Communications · 2024
Typearticle
Languageen
FieldMedicine
TopicMonoclonal and Polyclonal Antibodies Research
Canadian institutionsPrevention of Organ FailureSt. Paul's Hospital
FundersNational Heart, Lung, and Blood InstituteNational Institutes of HealthNational Institute of Allergy and Infectious DiseasesU.S. Department of Veterans AffairsU.S. Department of Health and Human Services
KeywordsGlycosylationImmunologyImmune systemAntibodyImmunoglobulin MGlycanBiologyImmunoglobulin GComplement systemVirologyGlycoproteinMolecular biologyBiochemistry

Abstract

fetched live from OpenAlex

The glycosylation of IgG plays a critical role during human severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection, activating immune cells and inducing cytokine production. However, the role of IgM N-glycosylation has not been studied during human acute viral infection. The analysis of IgM N-glycosylation from healthy controls and hospitalized coronavirus disease 2019 (COVID-19) patients reveals increased high-mannose and sialylation that correlates with COVID-19 severity. These trends are confirmed within SARS-CoV-2-specific immunoglobulin N-glycan profiles. Moreover, the degree of total IgM mannosylation and sialylation correlate significantly with markers of disease severity. We link the changes of IgM N-glycosylation with the expression of Golgi glycosyltransferases. Lastly, we observe antigen-specific IgM antibody-dependent complement deposition is elevated in severe COVID-19 patients and modulated by exoglycosidase digestion. Taken together, this work links the IgM N-glycosylation with COVID-19 severity and highlights the need to understand IgM glycosylation and downstream immune function during human disease.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.007

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0020.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.042
GPT teacher head0.385
Teacher spread0.343 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations28
Published2024
Admission routes1
Has abstractyes

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