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Record W4390790136 · doi:10.1016/j.clim.2024.109901

Favipiravir induces HuNoV viral mutagenesis and infectivity loss with clinical improvement in immunocompromised patients

2024· article· en· W4390790136 on OpenAlexaff
Alexandra Y. Kreins, Emma Roux, Juanita Pang, Iek Cheng, Oscar Charles, Sunando Roy, Reem Mohammed, Stephen Owens, David M. Lowe, Rachel C. Williams, Evey Howley, Timothy Best, E. Graham Davies, Austen Worth, Caroline Solas, Joseph F. Standing, Richard A. Goldstein, Joana Rocha‐Pereira, Judith Breuer

Bibliographic record

VenueClinical Immunology · 2024
Typearticle
Languageen
FieldMedicine
TopicViral gastroenteritis research and epidemiology
Canadian institutionsInstitute of Infection and Immunity
FundersMedical Research CouncilUCLH Biomedical Research CentreNIHR BioResourceManchester Biomedical Research CentreVlaamse regeringRosetrees TrustUniversity College London Hospitals NHS Foundation TrustFonds Wetenschappelijk OnderzoekNational Institute for Health and Care ResearchGreat Ormond Street Hospital CharityKU LeuvenGreat Ormond Street Hospital for ChildrenWellcome Trust
KeywordsFavipiravirInfectivityVirologyBiologyVirusSerial passageRNA-dependent RNA polymeraseNitazoxanideMutagenesisAntiviral drugRNAMutationMedicineGeneticsDiseaseImmunologyGeneCoronavirus disease 2019 (COVID-19)Infectious disease (medical specialty)

Abstract

fetched live from OpenAlex

Chronic human norovirus (HuNoV) infections in immunocompromised patients result in severe disease, yet approved antivirals are lacking. RNA-dependent RNA polymerase (RdRp) inhibitors inducing viral mutagenesis display broad-spectrum in vitro antiviral activity, but clinical efficacy in HuNoV infections is anecdotal and the potential emergence of drug-resistant variants is concerning. Upon favipiravir (and nitazoxanide) treatment of four immunocompromised patients with life-threatening HuNoV infections, viral whole-genome sequencing showed accumulation of favipiravir-induced mutations which coincided with clinical improvement although treatment failed to clear HuNoV. Infection of zebrafish larvae demonstrated drug-associated loss of viral infectivity and favipiravir treatment showed efficacy despite occurrence of RdRp variants potentially causing favipiravir resistance. This indicates that within-host resistance evolution did not reverse loss of viral fitness caused by genome-wide accumulation of sequence changes. This off-label approach supports the use of mutagenic antivirals for treating prolonged RNA viral infections and further informs the debate surrounding their impact on virus evolution.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.002
metaresearch head score (Gemma)0.001
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.274
Threshold uncertainty score0.818

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0020.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0000.000
Science and technology studies0.0000.001
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.002
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.044
GPT teacher head0.401
Teacher spread0.357 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations17
Published2024
Admission routes1
Has abstractyes

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