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Record W4392108825 · doi:10.3389/fmicb.2024.1357797

Microbiomes of Thalassia testudinum throughout the Atlantic Ocean, Caribbean Sea, and Gulf of Mexico are influenced by site and region while maintaining a core microbiome

2024· article· en· W4392108825 on OpenAlexaff
Kelly Ugarelli, Justin E. Campbell, O. Kennedy Rhoades, Calvin J. Munson, Andrew H. Altieri, James G. Douglass, Kenneth L. Heck, Valerie J. Paul, Savanna C. Barry, Lindsey Christ, James W. Fourqurean, Thomas K. Frazer, Samantha T. Linhardt, Charles W. Martin, Ashley M. McDonald, Vivienne A. Main, Sarah A. Manuel, Candela Marco‐Méndez, Laura K. Reynolds, Alex Rodriguez, Lucia M. Rodriguez Bravo, Yvonne Sawall, K. D. Smith, William L. Wied, Chang Jae Choi, Ulrich Stingl

Bibliographic record

VenueFrontiers in Microbiology · 2024
Typearticle
Languageen
FieldEnvironmental Science
TopicCoastal wetland ecosystem dynamics
Canadian institutionsUniversity of British Columbia
FundersFlorida International UniversityNational Science Foundation
KeywordsThalassia testudinumMicrobiomeBiologyMetagenomicsTaxonEcologyAmplicon sequencingMicrobial population biologyEcosystemSeagrass16S ribosomal RNABacteria

Abstract

fetched live from OpenAlex

Plant microbiomes are known to serve several important functions for their host, and it is therefore important to understand their composition as well as the factors that may influence these microbial communities. The microbiome ofThalassia testudinumhas only recently been explored, and studies to-date have primarily focused on characterizing the microbiome of plants in a single region. Here, we present the first characterization of the composition of the microbial communities ofT. testudinumacross a wide geographical range spanning three distinct regions with varying physicochemical conditions. We collected samples of leaves, roots, sediment, and water from six sites throughout the Atlantic Ocean, Caribbean Sea, and the Gulf of Mexico. We then analyzed these samples using 16S rRNA amplicon sequencing. We found that site and region can influence the microbial communities ofT. testudinum, while maintaining a plant-associated core microbiome. A comprehensive comparison of available microbial community data fromT. testudinumstudies determined a core microbiome composed of 14 ASVs that consisted mostly of the family Rhodobacteraceae. The most abundant genera in the microbial communities included organisms with possible plant-beneficial functions, like plant-growth promoting taxa, disease suppressing taxa, and nitrogen fixers.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.067
Threshold uncertainty score0.133

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0010.001
Science and technology studies0.0010.000
Scholarly communication0.0010.000
Open science0.0000.001
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0010.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.007
GPT teacher head0.213
Teacher spread0.207 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations5
Published2024
Admission routes1
Has abstractyes

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Same venueFrontiers in MicrobiologySame topicCoastal wetland ecosystem dynamicsFrench-language works237,207