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Record W4392137323 · doi:10.1016/j.aehs.2024.01.006

Pericytes express markers of cellular proliferation without expansion of the pool in response to exercise-induced skeletal muscle damage

2024· article· en· W4392137323 on OpenAlexaff
Joshua P. Nederveen, Katherine Manta, Donald Xhuti, Sean Y. Ng, Stephen A. Fortino, Tim Snijders, Sophie Joanisse, Dinesh Kumbhare, Hubert J.M. Smeets, Florence H. J. van Tienen, Gianni Parise

Bibliographic record

VenueAdvanced Exercise and Health Science · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicMuscle Physiology and Disorders
Canadian institutionsUniversity of TorontoMcMaster UniversityMcMaster University Medical Centre
Fundersnot available
KeywordsCell biologySkeletal muscleBiologyAnatomy

Abstract

fetched live from OpenAlex

Muscle-resident stromal cells, known as pericytes, have been shown to support muscle repair and/or regeneration. However, the extent to which pericytes respond during skeletal muscle repair in humans remains relatively unknown. The purpose of this study was to evaluate the pericyte response following damage-inducing eccentric muscle contractions. Healthy, young men (21.5 ± 0.5 years, n = 22), performed maximal muscle lengthening contractions via an isokinetic dynamometer. Muscle biopsies from the vastus lateralis were taken at baseline (Pre) and, 6 h-, 24 h-, 72 h-, 96 h following exercise-induced damage. Muscle-resident NG2 + pericyte content remained unchanged over time (p > 0.05), but the number of pericytes actively proliferating (Ki67 + /NG2 + ) was significantly (p < 0.05) elevated 24 h following damage. NG2 protein and associated mRNA expression of CSPG4 were significantly (p < 0.05) increased 24 h post-exercise. mRNA expression of signaling factors, related to pericyte mobility and activation including TNFa , CD248 and CXCR4 were significantly upregulated at 24 h post-exercise (p < 0.05). Taken together, muscle injury promotes the upregulation of nuclear proteins associated with proliferation of NG2 + pericytes and increases pericyte associated mRNA expression.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.202
Threshold uncertainty score0.304

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0010.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.012
GPT teacher head0.305
Teacher spread0.293 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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