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Record W4392299470 · doi:10.1101/2024.02.28.24303461

<i>APOL1</i> variants affect APOL1 plasma protein concentration: a UK Biobank study

2024· preprint· en· W4392299470 on OpenAlexaff
Walt E. Adamson, Harry Noyes, John Ogunsola, Rulan S. Parekh, Anneli Cooper, Annette MacLeod

Bibliographic record

VenuemedRxiv · 2024
Typepreprint
Languageen
FieldMedicine
TopicRenal Diseases and Glomerulopathies
Canadian institutionsSickKids FoundationHospital for Sick ChildrenWomen's College HospitalUniversity of Toronto
FundersUK Research and InnovationWellcome Trust
KeywordsBiobankBiologyGenome-wide association studyEthnic groupCohortGeneticsPhenotypeMedicineGenotypeGeneSingle-nucleotide polymorphismInternal medicine

Abstract

fetched live from OpenAlex

Abstract The APOL1 gene is associated with chronic kidney disease progression. Circulating APOL1 protein levels are likely to play a critical role. Using UK Biobank data from 43,330 participants with APOL1 protein concentration levels, we found that individuals self-reporting as Black or Black British individuals had significantly higher serum APOL1 levels than all other ethnicities. To investigate the genetic factors underlying this difference, we explored the impact of APOL1 genotypes and other potential modifiers on circulating APOL1 protein levels. We analysed APOL1 protein concentration data in 1,050 UK Biobank participants of recent sub-Saharan African ancestry, focusing on the APOL1 G1, G2, and N264K variants. APOL1 concentration showed a clear genotype-dependent effect: individuals with the G0/G0 genotype had the lowest levels, heterozygotes (G0/G1 and G0/G2), had intermediate levels, and individuals with the G2/G2 genotype had the highest levels, demonstrating a dose-dependent relationship. The N264K variant reduced protein levels on a G2 background (p = 6 x 10 −5 ). However, even after accounting for genotype, APOL1 protein levels in Black or Black British individuals was still higher than other ethnicities. A genome-wide association study on this population identified no genome-wide significant loci, other than the APOL1 gene itself (p = 3 x 10 −155 ) associated with APOL1 protein levels. Findings confirm APOL1 genotype as the major genetic determinant of circulating protein levels and provide new insights into the potential phenotypic effects of the G1, G2, and N264K variants.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.004
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: Observational
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.026
Threshold uncertainty score0.051

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.004
Meta-epidemiology (narrow)0.0010.001
Meta-epidemiology (broad)0.0010.000
Bibliometrics0.0010.002
Science and technology studies0.0010.001
Scholarly communication0.0010.000
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0040.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.022
GPT teacher head0.296
Teacher spread0.274 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2024
Admission routes1
Has abstractyes

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