Abstract 2268 Protein phosphatase SLP1 is a novel regulator of chloroplastic metabolism
Bibliographic record
Abstract
Photosynthesis is one of the most important biological phenomena on the planet. One regulatory mechanism for this process is the post-translational modification of proteins involved in catalyzing the light (in)dependent reactions occurring at the chloroplast thylakoid membranes and stroma. Like in all other eukaryotes, protein phosphorylation is the most prevalent post-translational modification in plants and is controlled by protein phosphatases and protein kinases. In the chloroplast only a few protein phosphatases are well characterized, with their substrates mostly being thylakoid membrane proteins. Following our group's bioinformatic discovery of chloroplast stroma localized protein phosphatase Shewanella-like phosphatase 1 (SLP1), our phosphoproteomics study suggests that SLP1 regulates the phosphorylation status of 126 proteins in the chloroplast. This gives SLP1 a supposedly widespread role and places it at the center of chloroplastic metabolism. Now having a list of putative targets, we sought out to identify how the phospho-status of select SLP1 target proteins may influence plant biology in Arabidopsis. Our group uses a combination of molecular biology, omics, and classical biochemistry to study the pleiotropic activity of SLP1. Furthermore, using a slp1-/- line, we have found SLP1 to be critical for regular starch, sugar, and lipid accumulation in Arabidopsis; we hypothesize the phospho-status of SLP1 target proteins is responsible for these observed phenotypes.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.001 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".