Additional file 1 of Dysregulation of Tweak and Fn14 in skeletal muscle of spinal muscular atrophy mice
Bibliographic record
Abstract
Additional file 1: Supplementary Figure 1. No overt dysregulation of Tweak and Fn14 in skeletal muscle of non-SMA hypomorphic Smn-depleted mice. qPCR analysis of Tweak (a), Fn14 (b), Pgc-1α (c), Mef2d (d), Glut-4 (e), HKII (f) and Klf15 (g) in triceps from post-natal day (P) 7 wild-type (WT), Smn2B/2B and Smn+/- mice. Normalized relative expressions are compared to WT. Data are mean ± SEM, n = 4-5 animals per experimental group, one-way ANOVA, Tukey’s multiple comparison test, * p < 0.05, ns = not significant. Supplementary Figure 2. Tweak and Fn14 are not dysregulated in denervated (nerve cut) muscles of pre-weaned mice. A sciatic nerve cut was performed on post-natal day (P) 7 WT FVB/N mice and both ipsilateral (nerve cut) and contralateral (control) TA muscles were harvested at P14. qPCR analysis of Tweak, Fn14, Pgc-1α, Mef2d, Glut-4, HKII and Klf15 in control and nerve cut TA muscles. Normalized relative expressions for each gene are compared to control muscle. Data are mean ± SEM, n = 7-11 animals per experimental group, two-way ANOVA, uncorrected Fisher’s LSD, ns = not significant. Supplementary Figure 3. Effect of varying Fc-TWEAK doses on disease progression in Smn-/-;SMN2 SMA mice. Smn-/-;SMN2 mice received daily subcutaneous injections of increasing doses of Fc-TWEAK (7.9, 15., 23.7 and 31.6 μg), starting at birth. a. Daily weights of untreated Smn-/-;SMN2 SMA mice and Smn-/-;SMN2 mice that received daily subcutaneous injections (starting at P0) of Fc-TWEAK (7.9, 15.8, 23.7 and 31.6 μg). Data are mean ± SEM, n = 5-10 animals per experimental group, two-way ANOVA, Sidak’s multiple comparison test. b. Survival curves of untreated Smn-/-;SMN2 SMA mice and Smn-/-;SMN2 mice that received daily subcutaneous injections (starting at P0) of Fc-TWEAK (7.9, 15.8, 23.7 and 31.6 μg). Data are presented as Kaplan-Meier survival curves, n = 5-10 animals per experimental group, Log-rank (Mantel-Cox). Supplementary Figure 4. Differential effect of Fc-TWEAK in skeletal muscle of wild type (WT) and Smn2B/- mice. WT and Smn2B/- SMA mice and received subcutaneous injections of Fc-TWEAK (15.8 μg) every 4 days (from post-natal day (P) 0 to P16) and skeletal muscles were harvested 3 hours post-injections. a-g. qPCR analysis of Fn14 (a), Tweak (b), Pgc-1α (c), Mef2d (d), Glut-4 (e), HKII (f) and Klf15 (g) in triceps of untreated and Fc-TWEAK-treated Smn2B/- mice. Normalized relative expressions for Fc-TWEAK-treated WT mice are compared to untreated WT mice and normalized relative expressions for Fc-TWEAK-treated Smn2B/- mice are compared to untreated Smn2B/- mice. Data are mean ± SEM, n = 3-5 animals per experimental group, two-way ANOVA, uncorrected Fisher’s LSD, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not significant. h. Quantification of NF-κB2 p100 protein levels normalized to total protein in the quadriceps of late-symptomatic (P18) Smn2B/- mice and age-matched WT animals. Images are representative immunoblots. Data are mean ± SEM, n = 3-5 animals per experimental group, unpaired t test, p = 0.0005 (WT), p = 0.0494. Supplementary Figure 5. Decreased MuRF-1 expression in skeletal muscle of P7 Fn14-/- mice. qPCR analysis of Atrogin-1 (a) and MuRF-1 (b) in quadriceps and triceps from post-natal day (P) 7 wild type (WT) and Fn14-/- mice. Normalized relative expressions are compared to WT. Data are mean ± SEM, n = 4 animals per experimental group, unpaired t test, p = 0.0164 (MuRF-1 quadriceps), p = 0.0283 (MuRF-1 triceps), ns = not significant.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.001 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.994 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".