Rapid Detection of Rice Fragrance Allele <i>badh2-E7</i> by Recombinant Polymerase Amplification (RPA)
Bibliographic record
Abstract
Fragrant rice is favored deeply by consumers due to the strong fragrance. Fragrant rice is mainly caused by the loss-of-function mutation of the Betaine aldehyde dehydrogenase 2 ( Badh2 ) gene in rice. Badh2-E7 , with 8 bp deletion and 3 bp substitution in exon 7 of Badh2 , is the main mutation allele used in fragrant rice breeding. In this study, a genotyping method named RPA-badh2-E7 for Badh2-E7 allele was designed. This method has the characteristics of rapid (completed amplification in 5 min), sensitivity (100-fold than conventional PCR), no strict amplification conditions required (25 °C~45 °C), and independent of PCR amplifier (only one thermostatic incubator is enough for amplification). This method greatly improved the efficiency of molecular marker-assisted selection of rice fragrant genes and breeding of fragrant rice varieties.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.001 |
| Meta-epidemiology (narrow) | 0.001 | 0.001 |
| Meta-epidemiology (broad) | 0.001 | 0.001 |
| Bibliometrics | 0.001 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.001 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.001 | 0.002 |
| Insufficient payload (model declined to judge) | 0.003 | 0.005 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".