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Record W4394950819 · doi:10.1101/2024.04.17.589749

From raw microalgae to bioplastics: conversion of <i>Chlorella vulgaris</i> starch granules into thermoplastic starch

2024· preprint· en· W4394950819 on OpenAlexaff
Alexandre Six, David Dauvillée, Christine Lancelon‐Pin, Alexandra Dimitriades-Lemaire, Ana Compadre, Carole Dubreuil, Pablo Álvarez, Jean‐François Sassi, Yonghua Li‐Beisson, Jean‐Luc Putaux, Nicolas Le Moigne, Gatien Fleury

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2024
Typepreprint
Languageen
FieldEnergy
TopicAlgal biology and biofuel production
Canadian institutionsCanadian Nautical Research Society
Fundersnot available
KeywordsStarchBioplasticChlorella vulgarisExtrusionThermoplasticFood sciencePotato starchRaw materialPolysaccharideBiomass (ecology)Materials scienceChemistryPulp and paper industryBotanyWaste managementBiologyAlgaeBiochemistryAgronomyOrganic chemistryComposite material

Abstract

fetched live from OpenAlex

Abstract Microalgae are emerging as a promising feedstock for bioplastics, with Chlorella vulgaris yielding significant amounts of starch. This polysaccharide is convertible into thermoplastic starch (TPS), a biodegradable plastic of industrial relevance. In this study, we developed a pilot-scale protocol for extracting and purifying starch from starch-enriched Chlorella vulgaris biomass. From 430.3 ± 0.5 g (dry weight - DW) of microalgae biomass containing 42.2 ± 3.4 % of starch, we successfully extracted 205.8 ± 1.2 g DW of purified starch extract containing 86.9 ± 3.0 % of starch, resulting in a final recovery yield of 98.5%. We have characterized this extracted starch and processed it into TPS using twin-screw extrusion and injection molding. Microalgal starch showed similar properties to those of native plant starch, but with smaller granules. We compared the mechanical properties of microalgal TPS with two controls, namely a commercial TPS and a TPS prepared from commercial potato starch granules. TPS prepared from microalgal starch showed a softer and more ductile behavior compared to the reference materials. This study demonstrates the feasibility of recovering high-purity microalgal starch on a pilot scale with high yields, and highlights the potential of microalgal starch for the production of TPS using industrially relevant processes. Graphical abstract

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.002
Threshold uncertainty score0.006

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.001
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.010
GPT teacher head0.217
Teacher spread0.207 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2024
Admission routes1
Has abstractyes

Explore more

Same venuebioRxiv (Cold Spring Harbor Laboratory)→Same topicAlgal biology and biofuel production→French-language works237,207→