Apirhabdus apintestini gen. nov., sp. nov., a member of a novel genus of the family Enterobacteriaceae, isolated from the gut of the western honey bee Apis mellifera
Bibliographic record
Abstract
A Gram-negative, motile, rod-shaped bacterial strain, CA-0114 T , was isolated from the midgut of a western honey bee, Apis mellifera . The isolate exhibited ≤96.43 % 16S rRNA gene sequence identity (1540 bp) to members of the families Enterobacteriaceae and Erwiniaceae . Phylogenetic trees based on genome blast distance phylogeny and concatenated protein sequences encoded by conserved genes atpD , fusA, gyrB , infB , leuS , pyrG and rpoB separated the isolate from other genera forming a distinct lineage in the Enterobacteriaceae . In both trees, the closest relatives were Tenebrionicola larvae YMB-R21 T and Tenebrionibacter intestinalis BIT-L3 T , which were isolated previously from Tenebrio molitor L., a plastic-eating mealworm. Digital DNA–DNA hybridization, orthologous average nucleotide identity and average amino acid identity values between strain CA-0114 T and the closest related members within the Enterobacteriaceae were ≤23.1, 75.45 and 76.04 %, respectively. The complete genome of strain CA-0114 T was 4 451669 bp with a G+C content of 52.12 mol%. Notably, the apparent inability of strain CA-0114 T to ferment d -glucose, inositol and l -rhamnose in the API 20E system is unique among closely related members of the Enterobacteriaceae . Based on the results obtained through genotypic and phenotypic analysis, we propose that strain CA-0114 T represents a novel species and genus within the family Enterobacteriaceae , for which we propose the name Apirhabdus apintestini gen. nov., sp. nov. (type strain CA-0114 T =ATCC TSD-396 T =DSM 116385 T ).
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".