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Repressor elements provide insights into tissue development and phenotypes in pigs

2024· article· en· W4395471512 on OpenAlexaff
Hang Liu, Yun Gao, Yongjun Tan, Lixian Wang, Chunhui Hou, Zhongyin Zhou, Ya‐Ping Zhang

Bibliographic record

Venue动物学研究 · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRNA Research and Splicing
Canadian institutionsUniversity of Toronto
FundersChinese Academy of SciencesNational Natural Science Foundation of China
KeywordsRepressorBiologyGenePhenotypeGeneticsRegulatory sequenceCell biologyEmbryonic stem cellRegulation of gene expressionComputational biologyGene expression

Abstract

fetched live from OpenAlex

Repressor elements play important roles in economic phenotypes of the pig, however, these elements remain poorly characterized. Here, we used H3K27me3, ATAC-seq and RNA-seq data derived from six tissues from three embryonic layers to identify 2 034 super repressor elements (SREs) and 22 223 typical repressor elements (TREs) in the pig genome. Among the identified repressor elements, many are shared by mesodermal and ectodermal tissues. SREs tightly regulate their target genes (within a specific genomic region, SREs act on a small number of target genes with strong effects) while the regulation by TREs is looser (TREs act on a wider range of target genes with weaker effects). Furthermore, in neuronal tissues, repressor element regulated genes start to be repressed during the stage of differentiation of stem cells to progenitor cells. Many regulatory elements were found to cooperate with each other (and have additive effects) to regulate the expression of KLF4. This study provides the first comprehensive pig repressor element map and should be a crucial reference for future studies on REs.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.413
Threshold uncertainty score0.250

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.291
Teacher spread0.280 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations1
Published2024
Admission routes1
Has abstractyes

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