A Portable, Rapid Isothermal Nucleic Acid Amplification System with Integrated Microfluidics for Pandemic Surveillance
Bibliographic record
Abstract
Integration of small-footprint cost-effective isothermal rapid DNA extraction, amplication, and detection systems is crucial to achieving a point-of-care pathogen detection system suitable for resource-limited locations.The herein system measures low-level uorescent signals in real-time during nucleic acid amplication, while maintaining the desired assay temperature on a low-power, portable footprint.A thermally stable microuidic device was implemented to mitigate thermocapillary effects and facilitate optical alignment for automated image capture and signal analysis.The system uses Loop-Mediated Isothermal Amplication with primers sensitive to broad strains and lineages of SARS-CoV-2 with viral loads corresponding to cycle thresholds in the range of 16 to 39.Among the 54 samples tested through optimizations, the platform yielded an 84% true positive rate.Preliminary investigations were conducted on developing an electromagnetic extraction process by printing a conductive coil around a microuidic channel.The extraction module was designed to be integrated with the detection system into a sample-to-results point-of-care system.I would like to thank Professor Ravi Prakash for his invaluable advice, help, and support as a supervisor throughout my graduate studies.The work achieved would not have been possible without his vision and expertise in the vast areas of science and engineering.I am extremely grateful to have been able to work on this high-impact project that has given me the experience to continue
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".