Abscisic acid controls sugar accumulation essential to strawberry fruit ripening via the <scp>FaRIPK1</scp> ‐ <scp>FaTCP7</scp> ‐ <i>FaSTP13/FaSPT</i> module
Bibliographic record
Abstract
SUMMARY Strawberry is considered as a model plant for studying the ripening of abscisic acid (ABA)‐regulated non‐climacteric fruits, a process in which sugar plays a fundamental role, while how ABA regulates sugar accumulation remains unclear. This study provides a direct line of physiological, biochemical, and molecular evidence that ABA signaling regulates sugar accumulation via the FaRIPK1‐FaTCP7‐ FaSTP13/FaSPT signaling pathway. Herein, FaRIPK1, a red‐initial protein kinase 1 previously identified in strawberry fruit, not only interacted with the transcription factor FaTCP7 (TEOSINTE BRANCHEN 1, CYCLOIDEA, and PCF) but also phosphorylated the critical Ser89 and Thr93 sites of FaTCP7, which negatively regulated strawberry fruit ripening, as evidenced by the transient overexpression (OE) and virus‐induced gene silencing transgenic system. Furthermore, the DAP‐seq experiments revealed that FvTCP7 bound the motif “GTGGNNCCCNC” in the promoters of two sugar transporter genes, FaSTP13 ( sugar transport protein 13 ) and FaSPT ( sugar phosphate/phosphate translocator ), inhibiting their transcription activities as determined by the electrophoretic mobility shift assay, yeast one‐hybrid, and dual‐luciferase reporter assays. The downregulated FaSTP13 and FaSPT transcripts in the FaTCP7‐OE fruit resulted in a reduction in soluble sugar content. Consistently, the yeast absorption test revealed that the two transporters had hexose transport activity. Especially, the phosphorylation‐inhibited binding of FaTCP7 to the promoters of FaSTP13 and FaSPT could result in the release of their transcriptional activities. In addition, the phosphomimetic form FaTCP7 S89D or FaTCP7 T93D could rescue the phenotype of FaTCP7‐OE fruits. Importantly, exogenous ABA treatment enhanced the FaRIPK1–FaTCP7 interaction. Overall, we found direct evidence that ABA signaling controls sugar accumulation during strawberry fruit ripening via the “FaRIPK1‐FaTCP7‐ FaSTP13 / FaSPT ” module.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.002 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".