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Record W4399708393 · doi:10.1101/2024.06.12.598728

Mechanisms of sustaining oxygen extraction efficiency in dragonfly nymphs during aquatic hypoxia

2024· preprint· en· W4399708393 on OpenAlexaff
Daniel J. Lee, Philip G. D. Matthews

Bibliographic record

VenuebioRxiv (Cold Spring Harbor Laboratory) · 2024
Typepreprint
Languageen
FieldEnvironmental Science
TopicPhysiological and biochemical adaptations
Canadian institutionsUniversity of British Columbia
Fundersnot available
KeywordsDragonflyNymphHypoxia (environmental)OxygenExtraction (chemistry)BiologyChemistryEcologyEnvironmental scienceZoologyFisheryOdonataChromatography

Abstract

fetched live from OpenAlex

Abstract Despite breathing water using their tidally ventilated rectal gills, dragonfly nymphs show a surprising ability to maintain oxygen (O 2 ) extraction from the water during hypoxia. However, an increase in convective O 2 transfer is insufficient to sustain aerobic demands by itself, which suggests that diffusive mechanisms must also be involved. This study examines the contributions of changing the O 2 partial pressure gradient (PO 2 ) and/or O 2 conductance across the rectal gill in maintaining O 2 extraction efficiency (OEE) of dragonfly nymphs during hypoxia. Data were collected using the same custom-designed respiro-spirometer described in a previous study with the addition of an implanted O 2 sensor to measure hemolymph PO 2 . Results show that the implantation of the O 2 sensor does not affect the respiratory and ventilatory response of nymphs to hypoxia. Hemolymph PO 2 fell from 6.3 ± 1.6 kPa at normoxia to 2.5 ± 0.6 kPa at 16.0 kPa, which resulted in the PO 2 diffusion gradient remaining statistically constant at these two water PO 2 s (17.5 ± 1.7 and 15.4 ± 0.7 kPa during normoxia and 16.0 kPa respectively). Beyond 16.0 kPa, a progressive reduction in hemolymph PO 2 was unable to sustain the diffusion gradient. Mathematical modeling revealed that while the addition of hemolymph PO 2 in tandem with ventilation frequency was able to elevate OEE during 16.0 kPa to that of normoxia, both were still insufficient during severe hypoxia and required an increase in O 2 conductance. Estimating the change in whole-gill conductance showed that nymphs are indeed increasing their conductance as the water becomes hypoxic, demonstrating a reliance on both diffusion gradient and O 2 conductance to enhance diffusive O 2 transfer in conjunction with convective mechanisms to maintain O 2 extraction during hypoxia.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.001
Threshold uncertainty score0.002

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.011
GPT teacher head0.215
Teacher spread0.203 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations2
Published2024
Admission routes1
Has abstractyes

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