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Record W4399870386 · doi:10.54097/2w49yh89

Descriptive comparison of ELISA and PCR methods in SARS-CoV-2 detection

2024· article· en· W4399870386 on OpenAlexaff
Shuwen Liu

Bibliographic record

VenueHighlights in Science Engineering and Technology · 2024
Typearticle
Languageen
FieldMedicine
TopicSARS-CoV-2 detection and testing
Canadian institutionsUniversity of New Brunswick
Fundersnot available
KeywordsVirologySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Coronavirus disease 2019 (COVID-19)Sars virusMedicineInfectious disease (medical specialty)Internal medicine

Abstract

fetched live from OpenAlex

The novel coronavirus, SARS-cov-2, is the causal agent of Covid-19, and allegedly to have a zoonotic origin. In 2019, it was reported in Wuhan, China, and declared pandemic by World Health Organization (WHO) between 2020 and 2023 soon after, to date, there are no known effective cure or treatments. Reverse transcription-polymerase chain reaction (RT-PCR) and enzyme-linked immunosorbent assay (ELISA) are both commonly used testing assays for SARS-cov-2 diagnosis, but only the RT-PCR recognized as the gold standard for the detection. Despite the better sensitivity and specificity, there are certain limitations RT-PCR possesses that make ELISA more preferable. This article presents a descriptive comparison of ELISA against RT-PCR in term of sensitivity and specificity. In addition, their advantages and disadvantages on these two methods were also discussed. The comparison showed better sensitivity and specificity on RT-PCR as expected, in the aspect of methodology, RT-PCR is more complex and time consuming relative to the ELISA counterpart, which making ELISA more favorable in large-scale implementations.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.008
metaresearch head score (Gemma)0.009
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.008
Threshold uncertainty score0.043

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0080.009
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0040.002
Science and technology studies0.0000.001
Scholarly communication0.0020.001
Open science0.0010.001
Research integrity0.0010.001
Insufficient payload (model declined to judge)0.0020.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.044
GPT teacher head0.363
Teacher spread0.318 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designObservational
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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