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Record W4401631622 · doi:10.22215/etd/2024-16007

Ghrelin’s Effects on Food-Seeking Behaviour in Female Mice

2024· dissertation· en· W4401631622 on OpenAlexaff
Kejah Bascon

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldNeuroscience
TopicRegulation of Appetite and Obesity
Canadian institutionsCarleton University
Fundersnot available
KeywordsGhrelinConditioned place preferenceEndocrinologyInternal medicineKnockout mousePreferenceAntagonistPsychologyFood preferenceAppetiteMedicineBiologyHormoneReceptorFood science

Abstract

fetched live from OpenAlex

This thesis investigates the effects of ghrelin and its receptor, the growth hormone secretagogue receptor (GHSR), on food reward and food-seeking behaviors in female mice.Previous research has primarily focused on male mice, demonstrating that ghrelin promotes food reward behaviors using the conditioned place preference (CPP) task.To determine if similar effects are observed in female mice, we conducted a series of CPP tasks with palatable food used as the reinforcer.In Experiment 1, we examined CPP responses in female mice after intraperitoneal (IP) injections of ghrelin (10nM/mouse) or saline under ad-lib conditions.Experiment 2 investigated the effects of the GHSR antagonist JMV 2959 (10 mg/kg) on CPP responses in both ad-lib and food-restricted conditions.In Experiment 3, we explored CPP responses to different doses of JMV 2959 (10 mg/kg, 20 mg/kg, and 30 mg/kg) under food-restricted conditions.Finally, Experiment 4 compared conditioned place preferences of GHS-R knockout and wildtype (WT) female mice in both food-restricted and ad-lib conditions.Results showed that the 10nM dose of ghrelin was not effective in enhancing a conditioned place preference to the compartment paired with the palatable food compared to controls.Ad-lib fed mice treated with JMV2959 showed reduced preference to the side paired with the palatable snack, but under food restricted conditions, JMV2959 treatment enhanced conditioned place preference to the palatable snack.Similarly, GHSR KO mice showed enhanced preference to the palatable snack but regardless of feeding status.These data suggest that the effects of peripheral ghrelin on motivated state may be complex and dependent on the feeding condition of the animal being tested.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: Bench or experimental
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.003
Threshold uncertainty score0.010

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.000
Science and technology studies0.0000.001
Scholarly communication0.0000.001
Open science0.0000.000
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0030.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.019
GPT teacher head0.288
Teacher spread0.269 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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