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Record W4401631765 · doi:10.22215/etd/2024-16062

Decoding Translational Control and Gene Expression

2024· dissertation· en· W4401631765 on OpenAlexaff
Sara Takalloo

Bibliographic record

Venuenot available
Typedissertation
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicRedox biology and oxidative stress
Canadian institutionsCarleton University
Fundersnot available
KeywordsCell biologyOxidative stressContext (archaeology)Reactive oxygen speciesBiologyTranscription factorMitochondrionOxidative phosphorylationGeneBiochemistry

Abstract

fetched live from OpenAlex

The optimal growth and functionality of organisms are fundamentally dependent on maintaining a robust physiological equilibrium within their cells.To sustain this balance, organisms have evolved intricate regulatory mechanisms designed to preserve intracellular homeostasis, even in the face of continuous variations in both intra-and extracellular environments.Within this context, oxidative stress presents a substantial challenge, significantly disrupting the cellular redox equilibrium and thus posing a threat to cellular stability.Reactive oxygen species (ROS) stand as one of the most potent forms of cellular stressors, embodying a paradoxical role within biological systems.They are both detrimental, causing oxidative damage to crucial cellular components, and beneficial, serving as essential contributors to innate immunity and cellular regulation.This duality underscores the importance of maintaining a critical balance in ROS production and neutralization, a balance that is foundational to cellular health.One of the most prevalent forms of ROS is hydrogen peroxide (H 2 O 2 ), primarily generated by the electron transport chain within the mitochondria.A multitude of genes have been identified as critical to the cellular detoxification process.Among these, yeast YAP1, homologous to the mammalian AP-1 type transcription factors, plays a pivotal role in oxidative detoxification.It achieves this by enhancing the expression of antioxidant genes in yeast, thereby actively contributing to the cell defense against oxidative stress.The current study uncovers new roles for COX5A, NPR3, CDA2, NCE102, and BCS1 in the context of H 2 O 2 -induced stress by demonstrating that their deletions result in increased sensitivity to oxidative stress condition.Further investigations linked the functions of these candidate genes directly to the regulation of YAP1 expression under oxidative stress.We demonstrated that these genes are crucial for facilitating the IRES-mediated translation of YAP1, especially under conditions where cap-dependent translation is compromised.This underscores their vital contribution to enhancing cellular defense mechanisms against oxidative stress.v I also want to thank the graduate office students for making studying fun and joyful.A special thanks to Caroline, Jess, Mina, Risham, Amarachi, and others for their friendship and spirit that made our academic journey memorable.Their presence made the tough days and challenges much more enjoyable. I also want to extend my gratitude to my

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.001
metaresearch head score (Gemma)0.004
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: none
Teacher disagreement score0.006
Threshold uncertainty score0.019

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0010.004
Meta-epidemiology (narrow)0.0010.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.001
Science and technology studies0.0010.002
Scholarly communication0.0020.002
Open science0.0000.001
Research integrity0.0010.002
Insufficient payload (model declined to judge)0.0060.002

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.006
GPT teacher head0.271
Teacher spread0.265 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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