Characterization of Oat Protein Fractions, Antioxidants and Tyrosinase Inhibitory Properties of their Hydrolysates
Bibliographic record
Abstract
Oat bran is a byproduct of oat milling that has garnered significant attention for its nutritional and functional properties, particularly its protein content.Apart from the nutritional purposes of oat, bioactive peptides from different cereals and grains have been reported to have significant antioxidant and tyrosinase inhibitory properties This study aimed to characterize the protein fractions derived from oat bran, hydrolyze them with digestive enzyme, evaluate the antioxidant potential and tyrosinase inhibition of their hydrolysates.The protein fractions, albumin, glutelin and globulin, were sequentially extracted from oat bran.The protein content of each fraction was 83.8%, 53.1% and 67.7%, respectively.Subsequently, the protein fractions were analyzed for their physicochemical properties, molecular weight distribution, and functional properties using analytical techniques such as SDS-PAGE and mass spectrometry.The molecular characteristics from SDS-PAGE analysis under reducing conditions showed that all fractions content subunits of 12 globulin at about 20 and 35 kDa but with differences in concentrations and intensity.In addition, the albumin fraction had a band at 10 kDa while the glutelin fraction had 52 and 150 kDa bands.The protein hydrolysates were prepared using digestive enzymes pepsin and In the journey of completing this thesis, I am deeply grateful to the Almighty.It is through His grace that I have found the strength to overcome challenges, the wisdom to navigate uncertainties, and the perseverance to reach this significant milestone.I would like to thank my supervisor, Professor Apollinaire Tsopmo for his support, patience, guidance, and supervision throughout this project.Special thanks to my parents Abdul Lateef and Abiola, your sacrifices, both seen and unseen, have provided me with the opportunity to pursue my academic aspirations."Ẹyin ni Olórùn ìkéjì mí láyé.".I am eternally grateful for the unconditional love, guidance, and the funny WhatsApp messages.To my siblings, Baba, Bariu, Iman and MM, the entertaining chaos that accompanied my academic journey, your teasing
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.001 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.001 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".