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Record W4402072935 · doi:10.5376/lgg.2024.15.0020

CRISPR/Cas9 Genome Editing in Legumes: Opportunities for Functional Genomics and Breeding

2024· article· en· W4402072935 on OpenAlex
Dandan Huang

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

venuePublished in a venue whose home country is Canada.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueLegume Genomics and Genetics · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicCRISPR and Genetic Engineering
Canadian institutionsnot available
Fundersnot available
KeywordsCRISPRGenome editingGenomicsFunctional genomicsBiologyGenomeComputational biologyGeneticsGene

Abstract

fetched live from OpenAlex

Legumes play a crucial role in global agriculture and food security, yet they face significant challenges in breeding for improved traits. This study explores the potential of CRISPR/Cas9 genome editing as a transformative tool in legume functional genomics and breeding. It begins by outlining the importance of legumes and the limitations of traditional breeding methods. The study then delves into the mechanisms and advantages of CRISPR/Cas9, highlighting its application in functional genomics, such as gene knockout and activation studies. A case study on drought tolerance in soybeans demonstrates the practical application of CRISPR/Cas9 in identifying and enhancing key traits. Furthermore, the study discusses the broad applications of this technology in improving biotic and abiotic stress resistance, enhancing quality traits, and accelerating the breeding process, including a detailed case study on disease resistance in chickpeas. The study also addresses the challenges and ethical considerations associated with CRISPR/Cas9, such as off-target effects and regulatory issues. Looking forward, the study explores future innovations and the integration of CRISPR/Cas9 into legume breeding programs, emphasizing its potential for sustainable agriculture and global food security. This study underscores the vast opportunities that CRISPR/Cas9 presents for advancing legume breeding and anticipates its growing impact on agricultural practices.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesMeta-epidemiology (narrow)
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.891
Threshold uncertainty score1.000

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.028
GPT teacher head0.278
Teacher spread0.249 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it