Study of the Amylolytic Activity of Thermophilic Bacteria Isolated from an Algerian Hot Spring (Azzaba, Skikda)
Bibliographic record
Abstract
Thermostable amylases are among the most widely used and desirable enzymes in the food industry.Indeed, they guarantee faster reactions at high temperatures, enhanced substrate solubility and reduced microbial contamination and cooling costs.The objective of this work is to study the amylase activity of three strains of aerobic thermophilic bacteria isolated from the hot spring of Hammam Salhine, located in the wilaya of Skikda, Algeria.The three extracellular amylase-producing strains were subjected to the quantification of amylase activity.They presented medium to high activity, with significantly the best production for the AS1 strain with an activity of 10.62 ± 1.289 U (p > 0.05).Monitoring the kinetics of AS1 amylase activity reveals that the maximum enzymatic activity was reached after 52 h with a value of 53.665 ± 2.534 U.The maximum growth was reached after 54 h of fermentation at an OD of 0.865 ± 0.081 at 600 nm.The study of the effect of the variation in physicochemical parameters on the activity of AS1 amylase extract shows that the enzymatic activity was maximal at a temperature of 100 • C, a pH of 8.0 and in the absence of NaCl.The amylase extract of this strain showed significant thermostability at 100 • C.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".