Phenological growth stages according to the BBCH-scale as a powerful tool for differentiating among CRISPR/Cas9 mutated lineages of the model plant <i>Setaria viridis</i> (L.) P. Beauv. (Poaceae)
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Bibliographic record
Abstract
In recent years, Setaria viridis (L.) P. Beauv. has increased its popularity as a model plant for genetic studies with C 4 monocotyledons. The BBCH-scale comprises a detailed phenotypic analysis, based on predefined growth stages, allowing for the construction of a comprehensive tool for morphological studies. We constructed a BBCH-scale for the ME034V wild-type accession and compared it with two independent mutants of the LONESOME HIGHWAY (LWH), which did not have noticeable morphological phenotypes. Our analysis showed that the main growth stages (0, 1, 2, and 7) showed homogeneity between the wild accession and the mutants. However, developmental changes between accessions were observable by comparing the secondaries of these main stages. There were significant differences between the wild accession and the mutants in relation to the main stages 4, 5, and 9. The total life cycle was reduced by 5 days in the svlhw-1 mutant. With a short life cycle like Setaria viridis, the observed changes can probably be attributed to genetic factors since the environmental conditions were strictly controlled. Thus, we showed that the BBCH-scale offers a powerful system to discriminate between Setaria viridis mutants that would otherwise be indistinguishable.
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Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.001 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it