Unraveling the Functional Role of R2R3-MYB Genes in Black Rice Anthocyanin Pathway: A CRISPR/Cas9 and Overexpression Study
Bibliographic record
Abstract
R2R3-OsMYBs play a pivotal role in the regulation of anthocyanin biosynthesis, which is responsible for the black pigmentation observed in rice. This paper is aimed to overview the functional mechanisms of these genes through the application of CRISPR/Cas9 gene editing and overexpression techniques. Recent studies have demonstrated the effectiveness of CRISPR/Cas9 in targeting key genes within the anthocyanin biosynthesis pathway, including OsF3'H , OsDFR , and OsLDOX , leading to significant alterations in anthocyanin content and seed color in rice. Moreover, the overexpression of R2R3-MYB genes, such as OsC1 , has been demonstrated to increase anthocyanin production and enhance oxidative stress tolerance in rice. Comparative studies in other plant species, including tomato and Nitraria sibirica , have further illuminated the diverse regulatory roles of R2R3-MYB TFs in anthocyanin biosynthesis. This review summarizes current findings to provide a comprehensive understanding of the genetic and molecular basis of anthocyanin regulation in black rice, offering insights into potential applications for crop improvement and functional food development.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.001 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".