Kurarinone, a Lavandulyl Flavanone from <i>Sophora flavescens</i>, Inhibits T-type Calcium Channels and Exerts Analgesic Effects in a Mouse Model of Inflammatory Pain
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
Kurarinone, the major lavandulyl flavanone identified in the roots of Sophora flavescens, has been reported to have different channel and transporter activity modulation capacities; nevertheless, its ability to block T-type channels and inflammatory pain activity have not been fully investigated. In this work, we used the whole-cell patch clamp technique to examine the ability of kurarinone to block T-type calcium channels. Kurarinone acted as a nonselective T-type channel antagonist that inhibited Cav3.2 channels expressed in tsA-201 cells with an IC 50 of 1.1 ± 0.3 μM and blocked native T-type channels in mouse dorsal root ganglion neurons. Transiently expressed Cav2.2 channels were also blocked. Molecular docking analysis predicted that the phenyl ring, lavandulyl, and hydroxyl groups of kurarinone interact directly with the pore domains of all three T-type calcium channels via hydrogen and hydrophobic interactions. Kurarinone administered intraperitoneally (10 mg/kg/i.p.) significantly inhibited phase II of formalin-induced nocifensive responses in mice. Furthermore, kurarinone reduced thermal hyperalgesia and mechanical hypersensitivity in mice injected with Complete Freund’s adjuvant (CFA) into the hind paw in an inflammatory pain mouse model. Taken together, our findings indicate that kurarinone has analgesic activity through blocking calcium channels.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.001 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.001 | 0.001 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.001 | 0.001 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it