Characterization of the <i>Plasmodium falciparum</i> homologue of Vps16, a member of the Vps-C tethering complex
Bibliographic record
Abstract
ABSTRACT The organelles of the apical complex (rhoptries, micronemes and dense granules) are critical for erythrocyte invasion by the malaria parasite Plasmodium falciparum . Though they have essential roles in the parasite lifecycle, the mechanisms behind their biogenesis are still poorly defined. The Class C Vps proteins Vps11, 16, 18 and 33 constitute the core of the CORVET and HOPS complexes implicated in vesicle tethering and fusion in the eukaryotic endolysosomal system. Work in the model apicomplexan Toxoplasma gondii has revealed that TgVps11 is essential for the generation of the apical complex. P. falciparum possesses all the four subunits of the Vps-C complex but their function is currently unknown. We here present an initial characterization of the P. falciparum orthologue of Vps16, a member of the Vps-C complex. Our structural predictions suggest that the structure of PfVps16 is similar to its other eukaryotic counterparts and that the binding region responsible for its interaction with PfVps33 is conserved. We next show that PfVps16 is expressed throughout the asexual erythrocytic cycle and that it is potentially associated with the rhoptries in schizont stage parasites. Finally, we present our unsuccessful attempts at studying its function using knock sideways.
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How this classification was reachedexpand
Full frame machine prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.
Distilled classifier scores by category (both heads)
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.001 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".