Vortex light field microscopy: 3D spectral single-molecule imaging with a twist
Why this work is in the frame
A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.
Bibliographic record
Abstract
3D single-molecule imaging reveals nanoscale structures in cell volumes but is limited by the need for spectrally distinct fluorophores. We address this limitation with vortex light field microscopy (VLFM), a 3D spectral single-molecule localization technique with 25 nm spatial and 3 nm spectral precision over a 4 µm depth of field. By modifying our previous single-molecule light field microscope with an azimuthally oriented prism array, we generated spectral disparity orthogonal to axial disparity, enabling simultaneous spatial and spectral localization on a single detector. We demonstrate VLFM with four-color 3D single-particle tracking and two-color 3D dSTORM imaging in fixed cells, successfully identifying dyes with spectral peaks just 15 nm apart. This shows VLFM’s potential for enhancing spatial biology workflows requiring highly multiplexed imaging.
Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it