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Record W4403527885 · doi:10.1016/j.csbj.2024.10.025

PIMMS-Dash: Accessible analysis, interrogation, and visualisation of high-throughput transposon insertion sequencing (TIS) data

2024· article· en· W4403527885 on OpenAlexfundno aff
Adam Blanchard, Adam Taylor, Andrew Warry, Freya Shephard, Alice Curwen, James A. Leigh, Richard D. Emes, Sharon A. Egan

Bibliographic record

VenueComputational and Structural Biotechnology Journal · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
Topicvaccines and immunoinformatics approaches
Canadian institutionsnot available
FundersBiotechnology and Biological Sciences Research CouncilDirectorate for Biological SciencesInternational Development Research Centre
KeywordsDashTransposable elementThroughputComputational biologySleeping Beauty transposon systemComputer scienceBiologyGeneticsGenomeTelecommunicationsOperating systemGeneWireless

Abstract

fetched live from OpenAlex

Motivation: Current methods for visualising and interrogating high-throughput transposon insertion mutagenesis sequencing (TIS) data requires a significant time investment in learning bioinformatics, often producing static figures that do not facilitate real time analysis. Summary: We have created an accessible web-based browser tool for visualisation and downstream analysis of high-throughput TIS data results generated by the PIMMS analysis pipeline. This includes multiple interactive and sortable tables to aid the user to identify genes of interest, enabling the user to gain a greater understanding of the genes contributing to fitness in their experimental work. PIMMS-Dash permits researchers, with any level of bioinformatics knowledge, to interrogate data sets and generate publication quality figures. Availability: PIMMS-Dash is freely available and is accessible online at https://pimms-dashboard-uon.azurewebsites.net and a Docker containerised version is available at https://github.com/Streptococcal-Research-Group/PIMMS-Dash to run locally.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Simulation or modeling · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.670
Threshold uncertainty score0.353

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.025
GPT teacher head0.290
Teacher spread0.265 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one teacher head, not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designSimulation or modeling
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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