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S1486 Pathogenic Crossroads: Viral Defense Genes as Modulators of Crohn's Disease Activity

2024· article· en· W4403722164 on OpenAlexaff
Kiandokht Bashiri, Abdiel Castillo Gurdian, Hussain Syed, Alireza Meighani, Akram I. Ahmad, Andrew L. Mason

Bibliographic record

VenueThe American Journal of Gastroenterology · 2024
Typearticle
Languageen
FieldBiochemistry, Genetics and Molecular Biology
TopicInflammatory Bowel Disease
Canadian institutionsUniversity of Alberta
Fundersnot available
KeywordsMedicineCrohn's diseaseImmunologyGeneDiseaseVirologyGeneticsInternal medicineBiology

Abstract

fetched live from OpenAlex

Introduction: Crohn's disease (CD) is a chronic inflammatory disorder of the gastrointestinal tract, characterized by alternating periods of remission and exacerbation. Understanding the interactions between host genetics and environmental factors, including potential viral triggers, is crucial for elucidating the underlying mechanisms of disease pathophysiology. Objective: This study aimed to investigate gene expression profiles that correlate with the Crohn's Disease Activity Index (SCDAI), with a specific focus on identifying molecular pathways that could indicate the involvement of viral elements in disease activity. Methods: We analyzed gene expression data from 389 intestinal biopsy samples collected from the ileum, provided by the SPARC dataset. Differential expression analysis was conducted between active and inactive disease states, followed by pathway analysis of differentially expressed genes using Reactome. Genes enriched in virus-related pathways were then selected for Pearson correlation analysis to assess associations between gene expression levels and SCDAI scores. Significance was determined through P-value adjustments using the Benjamini-Hochberg method to control the False Discovery Rate (FDR). Results: Significant correlations with SCDAI scores were found among genes involved in viral defense mechanisms, including TRIM7 (r: 0.157, FDR: 0.0215), FCN3 (r: 0.156, FDR: 0.0215), TLR2 (r: 0.139, FDR: 0.0394), APOBEC3A (r: 0.156, FDR: 0.0215), MLKL (r: 0.142, FDR: 0.0393), DDIT4 (r: 0.215, FDR: 0.000891), and OASL (r: 0.154, FDR: 0.0215). These genes participate in critical antiviral response pathways, suggesting that viral elements may significantly influence the exacerbation and modulation of inflammatory processes in Crohn's disease. Conclusion: Our findings underscore a significant link between viral defense mechanisms and the activity of Crohn's disease, highlighting the potential for viral infections to impact disease progression. This study contributes to a deeper understanding of Crohn's disease pathophysiology and emphasizes the importance of considering viral elements as a contributing factor in its dynamics (see Figure 1).Figure 1.: Network graph showing selected gene correlations with SCDAI and each other.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

How this classification was reachedexpand

Full frame machine prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. The Gemma side is a direct model label for every work in the frame, read from the title-only record. The Codex side is a classifier learned from the 10,348 direct Codex labels and calibrated to design-weighted sample rates; fields without enough sample support carry no Codex call. Candidate is the union of the two sides; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.001
Version: metacan-v3-hybrid-931329e0061cValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Bench or experimental · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.008
Threshold uncertainty score0.028

Distilled classifier scores by category (both heads)

CategoryCodexGemma
Metaresearch0.0000.001
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0010.001
Bibliometrics0.0010.001
Science and technology studies0.0000.000
Scholarly communication0.0010.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0080.001

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.005
GPT teacher head0.242
Teacher spread0.237 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it

Classification

machine, unvalidated

Machine predicted; a candidate call from one source (direct Gemma or distilled Codex), not a consensus.

The models applied no category: nothing in the taxonomy fit this work.
Study designBench or experimental
Domainnot available
GenreEmpirical

How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".

Quick stats

Citations0
Published2024
Admission routes1
Has abstractyes

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