Comparison of Physiological Stress Indices in Anesthetized and Manually Restrained Leopard Sharks, Triakis semifasciata
Bibliographic record
Abstract
Background: Leopard sharks (Triakis semifasciata) are abundant, coastal, eastern Pacific, mesopredatory sharks and are frequently managed in aquariums and zoos. Medical examinations are a routine part of good husbandry practices, but the handling protocols vary greatly between facilities. In this study, we compared the physiological stress responses of T. semifasciata associated with manual restraint and sedated handling under parallel holding and handling procedures in a 10 min interval. Methods: Blood was collected and analyzed for the hematocrit (Hct), lactate, glucose, beta-hydroxybutyrate (β-HB), pH, blood gasses, 1α-hydroxycorticosterone (1α-OHB), and corticosterone (B). Results: Overall, a minimal physiological stress response was observed in both groups. However, statistically significant increases in the PCO2, β-HB and lactate occurred, while the pHTC, SO2, and BE decreased over time in the manual-restraint group. In the sedated group, the B, PCO2, and lactate increased while the pHTC, SO2, and base excess (BE) decreased over time. When comparing treatments, the sedated group had a higher B and PCO2 TC and a lower pHTC, BE, and SO2 than the manual-restraint group at the second time point. Conclusions: This study indicates that manual restraint is comparable to the described sedation in T. semifasciata for minimally invasive procedures. The overall results show that this species had low physiological stress responses to both methods.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.000 |
| Science and technology studies | 0.000 | 0.001 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.001 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".