Early mucosal responses in common carp (Cyprinus carpio) to the infection of Aeromonas hydrophila
Bibliographic record
Abstract
Common carp ( Cyprinus carpio ), an economically important freshwater fish species, is worldwide farmed especially in Europe and Asia. The current high-density intensive rearing way leading to a high susceptibility to various pathogens, and Aeromonas hydrophila is one of the most frequently encountered bacteria, causing huge economic losses to the common carp industry. The mucosal barrier of fish constitutes the first line of defense against various pathogens, and some level of inter-connectivity exists among teleost mucosal tissues. However, the molecular basis for a common mucosal immune response at multiple sites or response at one site but during different timepoints following stimulation remains to be studied. In this study, we examined and systematically analyzed a total of 144 transcriptome files from three primary common carp mucosal tissues at four early timepoints following the infection of A. hydrophila . A total of 410 unigenes were significantly differentially expressed in all tested mucosal tissues. Through GO enrichment and KEGG pathway analysis, 28 key genes which might played critical roles in the common mucosal immune response of common carp during the early stage of bacterial infection were identified. Further WGCNA analysis showed that, besides the common response, three mucosal tissues exhibited tissue-specific gene regulatory network. Meanwhile, candidate hub genes in each tissue were identified, including trim3 , rpl14 , tln2 , itpr1 , and others. Our results will provide a fundamental basis for understanding the molecular mechanisms of teleost mucosal immunity, and might suggest strategies for developing novel teleost mucosal vaccines in aquaculture. • 144 samples from primary mucosal tissues at early timepoints following bacterial infection were sequenced and analyzed. • 28 key genes were identified which played critical roles in the early mucosal immune response of common carp. • 15 tissue-specific mucosal immune related hub genes were identified by combining WGCNA and co-expression network analysis.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.000 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".