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Record W4403990713 · doi:10.5376/gab.2024.15.0028

Harnessing Gene Editing Tools to Study ASFV Pathogenesis

2024· article· en· W4403990713 on OpenAlex
Xiaofang Lin

Why this work is in the frame

A frame that forgets how it found something cannot be audited. These are the routes that admitted this work.

venuePublished in a venue whose home country is Canada.
no affNo Canadian affiliation: this work is invisible to an affiliation-only frame.
No Canadian affiliation. An affiliation-only frame, the usual design, would never have seen this work. It is one of the works that make the case for inverting the frame.

Bibliographic record

VenueGenomics and Applied Biology · 2024
Typearticle
Languageen
FieldAgricultural and Biological Sciences
TopicAnimal Disease Management and Epidemiology
Canadian institutionsnot available
Fundersnot available
KeywordsGenome editingPathogenesisBiologyGeneticsGeneComputational biologyComputer scienceCRISPRImmunology

Abstract

fetched live from OpenAlex

The study utilizes advanced gene editing tools, specifically the CRISPR/Cas9 system, to investigate the pathogenesis of African Swine Fever Virus (ASFV) by creating recombinant virus strains with targeted gene deletions. The study successfully demonstrated the application of CRISPR/Cas9 to delete key immune response modulation genes (A238L, EP402R, and 9GL) in ASFV. The reconstituted virus exhibited similar replication kinetics to the parent virus, indicating that these genes can be modified with low frequency. Additionally, the use of CRISPR/Cas9 significantly accelerated the production of recombinant ASFV strains, reducing the time required from several months to less than two months. The study also highlighted the potential of CRISPR/Cas12a for sensitive and specific detection of ASFV, which could be crucial for on-site diagnostics and control of ASF outbreaks. The findings underscore the utility of CRISPR/Cas9 and CRISPR/Cas12a systems in both the study of ASFV pathogenesis and the development of rapid diagnostic tools. These advancements could pave the way for more effective control measures and the potential development of live-attenuated vaccines for ASFV.

Fetched live from OpenAlex and de-inverted. Abstracts are not stored in this database: the inverted indexes are 8.6 GB of the frame’s 9.3 GB of text, and the host has 13 GB free.

Full frame distilled prediction

Teacher imitation

Not calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.

metaresearch head score (Codex)0.000
metaresearch head score (Gemma)0.000
Version: codex-gemma-dda1882f352aValidation status: machine_predicted_unvalidated
Candidate categoriesnone
Consensus categoriesnone
DomainCandidate signal: none · Consensus signal: none
Study designCandidate signal: Observational · Consensus signal: none
GenreCandidate signal: Empirical · Consensus signal: Empirical
Teacher disagreement score0.778
Threshold uncertainty score0.237

Codex and Gemma teacher scores by category

CategoryCodexGemma
Metaresearch0.0000.000
Meta-epidemiology (narrow)0.0000.000
Meta-epidemiology (broad)0.0000.000
Bibliometrics0.0000.000
Science and technology studies0.0000.000
Scholarly communication0.0000.000
Open science0.0000.000
Research integrity0.0000.000
Insufficient payload (model declined to judge)0.0000.000

Machine scores (provisional)

The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.

Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.

Opus teacher head0.045
GPT teacher head0.259
Teacher spread0.214 · how far apart the two teachers sit on this one work
Validation statusscore_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from it