Characterization and genome analysis of two Aeromonas phages isolated from various sources related to chilled chicken
Bibliographic record
Abstract
Aeromonas is a primary spoilage bacterium found in chilled meat and serves as a significant pathogenic and spoilage agent in fish culture. Bacteriophages present a viable alternative to antibiotics for the targeted elimination of Aeromonas , minimizing environmental stress. In this study, two Aeromonas phages, P36 and P40, were isolated from samples collected in the slaughtering environment and from chilled chicken products. Both P36 and P40 exhibit the identical host range. They have optimal multiplicities of infection (MOI) of 0.001, with lysis times of 120 minutes and 180 minutes, respectively. These phages demonstrate stable survival within a temperature range of 4-40°C and a pH range of 5-11. Morphological and genomic analyses indicate that the two Aeromonas phages belong to the order Caudovirales , family Podoviridae , subfamily Autographivirinae . The genomes of the two phages were composed of linear double-stranded DNA (dsDNA) with lengths of 41,454 bp and 41,484 bp, respectively. Notably, there were no coding genes associated with safety factors such as virulence, antibiotic resistance, or lysogenicity present in the genomes. This finding indicates that the two phages possess potential applications in food safety and therapeutic contexts. • Two Aeromonas phages were isolated from slaughterhouses and chilled chickens. • Two lytic phages are classified in family Podoviridae , subfamily Autographivirinae. • Their genomes do not contain any known coding genes that present safety concerns.
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How this classification was reachedexpand
Full frame distilled prediction
Teacher imitationNot calibrated prevalence, not ground truth. Human validation pending. Learned from the 10,348 direct Codex labels and 10,348 direct Gemma labels. Candidate is the union of thresholded teacher heads; consensus is their intersection. These outputs are machine_predicted_unvalidated and are not human labels or direct frontier model labels.
Codex and Gemma teacher scores by category
| Category | Codex | Gemma |
|---|---|---|
| Metaresearch | 0.000 | 0.000 |
| Meta-epidemiology (narrow) | 0.000 | 0.000 |
| Meta-epidemiology (broad) | 0.000 | 0.000 |
| Bibliometrics | 0.000 | 0.001 |
| Science and technology studies | 0.000 | 0.000 |
| Scholarly communication | 0.000 | 0.000 |
| Open science | 0.000 | 0.000 |
| Research integrity | 0.000 | 0.000 |
| Insufficient payload (model declined to judge) | 0.004 | 0.000 |
Machine scores (provisional)
The two teacher heads of the student model, read on this work. A score orders the frame for review; it never asserts a category, and the validation status ships verbatim with every row.
Baseline scores from an immature model (maturity gate not passed, 7 training rounds). Scores rank; they never assert a category.
score_only:v0-immature-baseline · verbatim from the scoring run: score_only means the number may rank works, and no category label ships from itClassification
machine, unvalidatedMachine predicted; a candidate call from one teacher head, not a consensus.
How this classification was reached, model by model and score by score, is at the end of the page under "How this classification was reached".